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Characterization of Angiotensin I-Converting Enzyme (ACE) inhibitory peptides produced in fermented camel milk (Indian breed) by Lactobacillus acidophilus NCDC-15

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Abstract

Fermented camel milk provides many health benefits like antidiabetic activity, anti-hypertensive activity etc. Fermented camel milk contains IPP or VPP rich ACE inhibitory peptides. The aim of this study was to spot the novel Angiotensin I-Converting Enzyme inhibitory peptides liberated by the potent proteolytic Lactobacillus acidophilus NCDC-15 from camel milk (Indian breed). NCDC-15 had exhibited maximum PepX activity (0.655) and ACE-inhibitory activity (78.33%) at 12 and 48 h of incubation at 37 °C respectively. Proteolytic activity was measured using o-phthaldialdehyde method and observed maximum (0.976 OD) at 2% of inoculation for 12 h of incubation at 37 °C. Water soluble extracts derived from fermented camel milk were ultrafiltered through 3 kDa, 5 kDa and 10 kDa membrane filters from which 3 kDa permeates (48.01% peptides production & 49.46% ACE-inhibition) and 10 kDa permeates (55.04% peptides production & 42.40% ACE-inhibition) had shown maximum peptides production and ACE-inhibitory activity. Overall, 24 peptides were identified from the samples of 3 kDa permeates [6 fractions (K1, L1, M1, N1, O1 and P1)] and 10 permeates [5 fractions (S, T, U, V and W)]. Novel peptide (AIGPVADLHI) was matched with k-casein in AHTPDB database and other peptides were also found matched with α and β-caseins of camel milk.

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All data generated or analysed during this study are included in this published article.

Abbreviations

ACE:

Angiotensin I-Converting Enzyme

AHTPDB :

Database of antihypertensive peptides

BLAST:

Basic local alignment search tool

EMS:

Enhanced mass spectra scan

EPI:

Enhanced product ion

ESI:

Electron spray ionization

Gly-Pro-pNA:

Glycyl-prolyl p-nitroanilide

HA :

Hippuric acid

HHL:

N-hippuryl-histidne-leucine

HL:

Histydyl-leucine

HPL :

High performance liquid chromatography

IDA:

Information dependent analysis

NCBI:

National Centre for Biotechnology Information

NCDC:

National Collection of Dairy Culture

OD:

Optical density

RP-LC/MS:

Reversed phase liquid chromatography mass spectrometry

PIR:

Protein information resources

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Acknowledgements

The Authors thankfully acknowledge the Plant Biotechnology Department, Anand Agricultural University, Anand, India for providing the LCMS facility for peptide sequencing.

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Authors and Affiliations

  1. Dairy Microbiology Department, SMC College of Dairy Science, Anand Agricultural University, Anand, Gujarat, 388110, India

    Divyang Solanki & Subrota Hati

  2. Department of Agricultural Biotechnology, Anand Agricultural University, Anand, Gujarat, 388110, India

    Amar Sakure

  3. School of Agriculture and Food Sciences, University of Queensland, Brisbane, Australia

    Sangeeta Prakash

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  1. Divyang Solanki
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  2. Amar Sakure
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  3. Sangeeta Prakash
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  4. Subrota Hati
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DS: Methodology, investigation, formal analysis, data curation, writing—original draft preparation. AS: data curation; formal analysis; methodology; project administration; resources; software; SP: data curation; formal analysis; writing—review and editing; methodology; SH: supervision, conceptualization, funding acquisition, investigation, project administration, resources, validation.

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Correspondence to Subrota Hati.

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Solanki, D., Sakure, A., Prakash, S. et al. Characterization of Angiotensin I-Converting Enzyme (ACE) inhibitory peptides produced in fermented camel milk (Indian breed) by Lactobacillus acidophilus NCDC-15. J Food Sci Technol 59, 3567–3577 (2022). https://doi.org/10.1007/s13197-022-05357-9

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