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Hydrolysis of milk-derived bioactive peptides by cell-associated extracellular peptidases of Streptococcus thermophilus

  • Applied microbial and cell physiology
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Abstract

The trend to confer new functional properties to fermented dairy products by supplementation with bioactive peptides is growing in order to encounter the challenge of health-promoting foods. But these functional ingredients have not to be hydrolysed by proteases of bacteria used in the manufacture of these products. One of the two yoghurt bacteria, Streptococcus thermophilus, has long been considered as weakly proteolytic since its only cell wall-associated subtilisin-like protease, called PrtS, is not always present. Nevertheless, a recent study pointed out a possible peptidase activity in certain strains. In this present study, the stability of milk-derived bioactive peptides, e.g. the anxiolytic peptide, αs1-CN-(f91-97), in the presence of two different S. thermophilus strains with PrtS+ or PrtS− phenotype was studied. Both strains appeared to be capable of hydrolysing the αs1-CN-(f91-97) and other bioactive peptides by recurrent removal of N-terminal residues. The hydrolysis was neither due to intracellular peptidases nor to HtrA protease. Results obtained showed that the observed activity originates from the presence at the surface of both strains of an extracellular aminopeptidase activity. Moreover, a cell wall-associated X-prolyl dipeptidyl peptidase activity was also highlighted when β-casomorphin-7 was used as substrate. All of these findings suggest that, in order to use fermented milks as vector of bioactive peptides, the stability of these bioactive peptides in this kind of products implies to carefully characterize the potential action of the surface proteolytic enzymes of S. thermophilus.

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Acknowledgments

Zeeshan Hafeez is highly thankful to the Higher Education Commission (HEC) of Pakistan for financial support.

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Authors and Affiliations

  1. Université de Lorraine, Unité de Recherche « Animal & Fonctionnalités des Produits Animaux » (UR AFPA), Équipe « Protéolyse & Biofonctionnalités des Protéines et des Peptides » (PB2P), Vandœuvre-lès-Nancy, 54506, France

    Zeeshan Hafeez, Céline Cakir-Kiefer, Jean-Michel Girardet, Clarisse Perrin, Annie Dary & Laurent Miclo

  2. INRA, Unité de Recherche « Animal & Fonctionnalités des Produits Animaux » (UR AFPA), USC 340, Vandœuvre-lès-Nancy, 54506, France

    Zeeshan Hafeez, Céline Cakir-Kiefer, Jean-Michel Girardet, Clarisse Perrin, Annie Dary & Laurent Miclo

  3. INRA, UMR 1253 Science et Technologie du Lait et de l’Œuf , Rennes, 35042, France

    Julien Jardin

  4. Agrocampus Ouest, UMR1253 Science et Technologie du Lait et de l’Œuf, Rennes, 35042, France

    Julien Jardin

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  1. Zeeshan Hafeez
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  2. Céline Cakir-Kiefer
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  3. Jean-Michel Girardet
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  7. Laurent Miclo
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Correspondence to Laurent Miclo.

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Hafeez, Z., Cakir-Kiefer, C., Girardet, JM. et al. Hydrolysis of milk-derived bioactive peptides by cell-associated extracellular peptidases of Streptococcus thermophilus . Appl Microbiol Biotechnol 97, 9787–9799 (2013). https://doi.org/10.1007/s00253-013-5245-7

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