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Transfer of HMW glutenin subunits from Aegilops kotschyi to wheat through radiation hybridization

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Abstract

High molecular weight glutenin subunits (HMWGS) are responsible for dough elasticity and bread making quality of bread wheat. Related wild non-progenitor species, Aegilops kotschyi possesses higher molecular weight x and y glutenin subunits than the bread wheat cultivars. A wheat-Aegilops substitution line with 1U chromosome was used for the transfer of (HMWGS) of 1U to wheat by using pollen radiation hybridization approach. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis profiling showed different patterns of allelic variations with either the presence or absence of HMWGS, Glu-1A (1, null), Glu-1B (7, 7 + 8, 17 + 18) and Glu-1D (5 + 10, 2 + 12, null). The pollen irradiated wheat-Aegilops derivatives, B-56-1-4-2, B-56-1-4-3, B-14-1 and B-14-2 with Glu1Ux and 1Uy and absence or presence of some Glu-1A and Glu-1B HMWGS showed high micro SDS sedimentation test (MST) values while B-16-1 and B-16-2 had moderate MST values and high protein content. However, B-58-3 with transfer of Glu-1Ux + 1Uy for Glu-1D showed very low MST values indicating that Glu-1Ux + 1Uy enhance MST value only in the presence of Glu1D HMWGS. The transfer/substitution of alien HMW-GS for Glu-1A and or Glu-1B loci only can lead to improved bread making quality of wheat.

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Acknowledgments

The financial support provided by Department of Biotechnology, Govt. of India (BT/AGR/Wheat Bioforti/PH-II/2010) is duly acknowledged. The authors also acknowledge Akal College of Agriculture for providing necessary infrastructure and research facilities for carrying out this work.

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Authors and Affiliations

  1. Akal College of Agriculture, Eternal University, Baru Sahib, Himachal Pradesh, 173101, India

    Jasmeet Singh, Imran Sheikh, Prachi Sharma, Rahul Kumar, Pritesh Vyas & H. S. Dhaliwal

  2. Department of Molecular Biology and Genetic Engineering, GB Pant University of Agriculture and Technology, Pant Nagar, Uttarakhand, 263145, India

    Priyanka Mathpal & Sundip Kumar

  3. Department of Biotechnology, Indian Institute of Technology, Roorkee, Uttarakhand, 247667, India

    Satish Kumar

  4. School of Life Sciences, Central University of Himachal Pradesh, Kangra, Himachal Pradesh, 176215, India

    Shailender Kumar Verma

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  1. Jasmeet Singh
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  2. Imran Sheikh
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  3. Prachi Sharma
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  4. Satish Kumar
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  5. Shailender Kumar Verma
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  6. Rahul Kumar
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  7. Priyanka Mathpal
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  8. Sundip Kumar
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  9. Pritesh Vyas
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  10. H. S. Dhaliwal
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Correspondence to H. S. Dhaliwal.

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Singh, J., Sheikh, I., Sharma, P. et al. Transfer of HMW glutenin subunits from Aegilops kotschyi to wheat through radiation hybridization. J Food Sci Technol 53, 3543–3549 (2016). https://doi.org/10.1007/s13197-016-2333-6

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  • DOI: https://doi.org/10.1007/s13197-016-2333-6

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