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Phytochemical study and antioxidant, antimicrobial and anticancer activities of Melanelia subaurifera and Melanelia fuliginosa lichens

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Abstract

The aim of this study was to investigate antioxidant, antimicrobial and anticancerous activity of Melanelia subaurifera and Melanelia fuliginosa. The phytochemical analysis was determined by HPLC–UV method. Antioxidant activity was evaluated by DPPH and reducing power assay while antimicrobial activity was determined by minimal inhibitory concentration. The cytotoxic activity was tested using MTT method. The method for quantification of 2′-O-methyl anziaic acid and lecanoric acid in these lichens using RF-HPLC was also developed and validated. The depsides (lecanoric acid, gyrophoric acid, atranorin, anziaic acid and 2′-O-methyl anziaic acid), and dibenzofurane (usnic acid) were identified in these lichens. The antioxidant activity (IC50) of lichens extracts ranged from 121.52 to 424.51 μg/ml. 2′-O-Methyl anziaic acid showed the highest antimicrobial activity with MIC ranging from 0.0625 to 1 mg/ml. M. subaurifera extract showed the highest cytotoxic activity against the tested cell lines (IC50 = 9.88 to 31.64 μg/ml).

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Acknowledgments

This work was financed in part by the Ministry of Science, Technology, and Development of the Republic of Serbia and was carried out within the framework of Project Nos. 173032, 175011 and 172015.

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Authors and Affiliations

  1. Department of Biology, Faculty of Science, University of Kragujevac, Kragujevac, 34000, Serbia

    Svetlana Ristić, Branislav Ranković & Marijana Kosanić

  2. Institute of Oncology and Radiology of Serbia, Belgrade, 11000, Serbia

    Tatjana Stanojković

  3. Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Nis, 18000, Serbia

    Slaviša Stamenković & Perica Vasiljević

  4. Department of Pharmacy, Faculty of Medicinal Sciences, University of Kragujevac, Kragujevac, 34000, Serbia

    Ivana Manojlović & Nedeljko Manojlović

Authors
  1. Svetlana Ristić
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  2. Branislav Ranković
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  3. Marijana Kosanić
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  4. Tatjana Stanojković
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  5. Slaviša Stamenković
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  6. Perica Vasiljević
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  7. Ivana Manojlović
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  8. Nedeljko Manojlović
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Correspondence to Marijana Kosanić.

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Ristić, S., Ranković, B., Kosanić, M. et al. Phytochemical study and antioxidant, antimicrobial and anticancer activities of Melanelia subaurifera and Melanelia fuliginosa lichens. J Food Sci Technol 53, 2804–2816 (2016). https://doi.org/10.1007/s13197-016-2255-3

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  • DOI: https://doi.org/10.1007/s13197-016-2255-3

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