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Molecular phylogeography and population genetic structure of the planktonic copepod Calanus sinicus Brodsky in the coastal waters of China

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Abstract

Planktonic copepod Calanus sinicus is the dominant meso-zooplankton in the Northwest Pacific Ocean. To better understand its population dynamics and phylogeographic patterns, 243 C. sinicus individuals were collected from seven locations across the shelf waters of China and its population genetics was studied by mitochondrial DNA cytochrome oxidase I (mtCOI) sequences analyses. Thirty-nine different sequences, or haplotypes, were detected with moderate haplotype diversity (h=0.749) and low nucleotide diversity (π=0.003) for all populations. The evolutionary divergence between geographic populations varied from 0.24% to 0.37%, indicative of very limited genetic differentiation. Visualized minimum spanning network (MSN) and phylogenetic analysis of all the detected haplotypes did not reveal any clear phylogeographic pattern. Furthermore, AMOVA data showed no significant spatial population differentiation existed among the individuals collected across China shelf waters. Pairwise F ST values showed that population collected from northwest of the East China Sea (ECS) displayed a low difference to other populations. Mismatch distribution analyses and neutrality tests indicated that C. sinicus might undergo a demographic/population expansion. No significant population genetic structuring was detected, indicating an extensive gene flow among the C. sinicus populations. Our results provide molecular evidence for the hypothesis that C. sinicus in the northwestern South China Sea in winter is transported from the East China Sea and the Yellow Sea by the China Coastal Current during the northeast monsoon period.

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Authors and Affiliations

  1. College of Environmental Science and Engineering, Ocean University of China, Qingdao, 266100, China

    Yousong Huang, Guangxing Liu & Xiaofeng Chen

  2. Key Laboratory of Marine Environment and Ecology of Ministry of Education, Ocean University of China, Qingdao, 266100, China

    Guangxing Liu

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  1. Yousong Huang
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Correspondence to Guangxing Liu.

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Foundation item: The National Natural Science Foundation of China under contract Nos 40876066 and 41076085; the National Basic Research Program of China under contract No. 2005CB422306.

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Huang, Y., Liu, G. & Chen, X. Molecular phylogeography and population genetic structure of the planktonic copepod Calanus sinicus Brodsky in the coastal waters of China. Acta Oceanol. Sin. 33, 74–84 (2014). https://doi.org/10.1007/s13131-014-0542-2

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