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Improving Curcumin Constraints with pH-Responsive Chitosan Based Graphene Oxide/Montmorillonite Nanohybrid Modified Agarose in Breast Cancer Therapy

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Abstract

Nanoparticles have promisingly served as therapeutic agents in drug delivery systems to supress side effects while improving the effectiveness of treatment to deadly cancers. The present study aims to fabricate chitosan/agarose/graphene oxide/montmorillonite nanocomposites to serve as drug carriers for the prolonged release of curcumin to suppress MCF7 breast cancer cells. Offering a range of advantages, including biocompatibility and pH-sensitivity, the nanocomposites were synthesized via a water-in-oil-in-water (W/O/W) emulsification technique. Characterization studies were conducted with FTIR and XRD analyses. Dynamic light scattering (DLS) analysis was conducted to measure the mean size of the produced nanoscale emulsions, with zeta potential assessments further performed to characterize the nanocomposites in terms of surface charge. FE-SEM imagery indicated the uniform and flat surface of the synthesized nanocomposites. MTT assay results showed that the nanocomposites were most toxic to MCF7 breast cancer cells. The occurrence of apoptosis to MCF7 breast cancer cells was evident from the flow cytometry findings. Implementing a dialysis procedure, the curcumin was found to be released at a higher rate in acidic media. Altogether, our findings proved that the designed CS/AG/GO/MMT@CUR nanocomposites could be a drug carrier for treating breast cancer cells.

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Notes

  1. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

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Funding

This work was supported by the Iran National Science Foundation (INSF).

Author information

Authors and Affiliations

  1. Department of Biotechnology, School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran

    Mariyeh Rajaei & Hamid Rashedi

  2. Department of Life Science Engineering, Faculty of New Science and Technologies, University of Tehran, Tehran, Iran

    Fatemeh Yazdian

  3. Pharmaceutical Sciences Research Center, The Institute of Pharmaceutical Sciences (TIPS), Tehran University of Medical Sciences, Tehran, Iran

    Mona Navaei-Nigjeh

  4. Department of Physics, Faculty of Sciences, University of Zabol, Zabol, 538-98615, Iran

    Abbas Rahdar

  5. Graduate Program in Genomic Sciences and Biotechnology, Catholic University of Brasília, Brasília, 71.966-700, Brazil

    Luiz Fernando Romanholo Ferreira

  6. Protein Research Center, Shahid Beheshti University, Tehran, GC, 1983963113, Iran

    Mehrab Pourmadadi

Authors
  1. Mariyeh Rajaei
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  2. Mehrab Pourmadadi
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  3. Hamid Rashedi
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  4. Fatemeh Yazdian
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  5. Mona Navaei-Nigjeh
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  6. Abbas Rahdar
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  7. Luiz Fernando Romanholo Ferreira
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Contributions

M.R.: data curation, formal analysis, methodology, investigation, visualization, writing-original draft preparation. M.P.: data curation, conceptualization, investigation, visualization, writing-original draft preparation, writing—reviewing and editing. H.R.: visualization, conceptualization, validation, resources, supervision, writing—reviewing and editing. F.Y.: visualization, validation, resources, writing—reviewing and editing. M.N-N.: visualization, validation, writing—reviewing and editing. A.R.: visualization, validation, resources, supervision, writing—reviewing and editing. L.F.R.F.: visualization, validation, writing—reviewing and editing.

Corresponding authors

Correspondence to Hamid Rashedi, Fatemeh Yazdian, Abbas Rahdar or Luiz Fernando Romanholo Ferreira.

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Highlights

• Chitosan-based nanomaterials enhance cancer therapy.

• Drug delivery system: chitosan, graphene oxide, agarose, and montmorillonite nanoparticles.

• NC encapsulation with CUR improves breast cancer treatment.

• Results confirm cytotoxicity, pH sensitivity, and sustained drug release against MCF7 cells.

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Rajaei, M., Pourmadadi, M., Rashedi, H. et al. Improving Curcumin Constraints with pH-Responsive Chitosan Based Graphene Oxide/Montmorillonite Nanohybrid Modified Agarose in Breast Cancer Therapy. BioNanoSci. (2024). https://doi.org/10.1007/s12668-024-01385-1

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  • DOI: https://doi.org/10.1007/s12668-024-01385-1

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