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Effects of Extraction Methods on the Physicochemical, Structural, Functional Properties and Biological Activities of Extracted Polysaccharides from Ononis natrix Leaves

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Abstract

Polysaccharides were extracted from Ononis natrix leaves using various methods, including hot water maceration (HWM), ultrasound-assisted extraction (USE), enzyme-assisted extraction (ENE), combined enzyme-ultrasound (EUS) and combined maceration-ultrasound (MUS) treatment. The extracted crude polysaccharides were characterized in terms of physicochemical, structural, techno-functional features, and biological activities. HWM extraction showed the highest yield (13.7 ± 0.3%), carbohydrate content (54.1 ± 1.4%), and uronic acid content (23.7 ± 0.8%) compared to other methods. The various polysaccharides were characterized using SEM, FT-IR, NMR, and TGA. All polysaccharides obtained using different methods present similar structural characteristics and exhibit outstanding techno-functional properties (solubility, water-holding capacity, and emulsifying properties). The antioxidant and antibacterial activities of polysaccharides were evaluated using DPPH radical-scavenging capacity, ferric reducing activity power (FRAP), iron chelating capacity, total antioxidant activity, and agar diffusion test, respectively. The results indicate that HWM polysaccharides present the best overall biological activities. It is thus concluded that polysaccharides from O. natrix leaves could be a promising natural source as functional foods or potential therapeutic agents.

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Acknowledgements

This researchworkwasconducted in the framework of PHC-Utique Program, (partenariat Hubert Curien « Utique » du Ministère de l’Europe et des Affaires Etrangères français et du Ministère de l’Enseignement et de la Recherche Scientifique tunisien) financed by CMCU (Comité mixte de coopération universitaire), grant N° : 19G0815.

Funding

This study was supported by the Ministry of Higher Education and Scientific Research (MESRS) in Tunisia.

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Authors and Affiliations

  1. Laboratory of Enzyme Engineering and Microbiology, National School of Engineering of Sfax (ENIS), University of Sfax, P.O. Box 1173, 3038, Sfax, Tunisia

    Nour Bhiri, Mohamed Hajji, Rim Nasri, Tayssir Mekki & Moncef Nasri

  2. Institut Européen Des Membranes, IEM, UMR 5635, Univ Montpellier, CNRS, ENSCM, Montpellier, France

    Nour Bhiri & Suming Li

Authors
  1. Nour Bhiri
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  2. Mohamed Hajji
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  3. Rim Nasri
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  4. Tayssir Mekki
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  5. Moncef Nasri
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  6. Suming Li
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Correspondence to Mohamed Hajji or Suming Li.

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Statement of Novelty

This work aimed to extract polysaccharides from the leaves of O. natrix using different methods such as hot water maceration (HWM), ultrasonic-assisted extraction (USE), enzyme-assisted extraction (ENE), combined enzyme-ultrasonic assisted extraction (EUS), and combined maceration-ultrasonic assisted extraction (MUS). The extracted polysaccharides were then investigated in terms of their physicochemical properties, structural characteristics, functional properties, antioxidant activities, and antibacterial activity against seven different bacteria strains. The results of this study may provide technical support and an experimental basis for the full valorization of the above-ground part of this Tunisian plant as a source of biopolymers in various fields.

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Bhiri, N., Hajji, M., Nasri, R. et al. Effects of Extraction Methods on the Physicochemical, Structural, Functional Properties and Biological Activities of Extracted Polysaccharides from Ononis natrix Leaves. Waste Biomass Valor (2024). https://doi.org/10.1007/s12649-024-02546-9

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