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Physicochemical properties and in vitro bioactivities of polysaccharides from lotus leaves extracted by different techniques and solvents

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Abstract

The present study aimed to investigate the effect of four kinds of extraction technologies and three solvents on the chemical and biological functions of water-soluble polysaccharides from lotus leaves (LLPs). Results revealed that the yields, basically chemical compositions, molecular weight distributions, compositional monosaccharides, FT-IR spectra, and apparent viscosities of LLPs varied by different technologies, especially by different solvents. All LLPs from lotus leaves possessed obvious biological properties, including antioxidant abilities and inhibitory effects against digestive enzymes. Especially, the lower molecular weight distribution and higher content of uronic acids were observed in polysaccharides extracted by deep eutectic solvent (DES) assisted extraction and DES-microwave assisted extraction, respectively, which might contribute to their stronger antioxidant abilities and inhibitory effects against digestive enzymes. Furthermore, the strong biological activities of alkali extracted polysaccharides were also closely associated with its low molecular weight and high content of proteins. Besides, polysaccharides extracted by pressurized water extraction exhibited strong bioactivities than that of LLPs extracted by other extraction technologies. These findings could provide fundamental knowledge to choose suitable processes for preparing LLPs with desired biological functions and extend the application of polysaccharides from lotus leaves in the functional food industry.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (31901690), and the Scientific Research Foundation of Chengdu University (2081921047).

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  1. Kang-Lin Feng and Ling Huang contributed equally to this work.

Authors and Affiliations

  1. Institute of Food Processing and Safety, College of Food Science, Sichuan Agricultural University, Ya’an, 625014, Sichuan, China

    Kang-Lin Feng, Ling Huang, Ding-Tao Wu, Fen Li & Wen Qin

  2. Key Laboratory of Coarse Cereal Processing, Ministry of Agriculture and Rural Affairs, Sichuan Engineering & Technology Research Center of Coarse Cereal Industralization, School of Food and Biological Engineering, Chengdu University, Chengdu, 610106, China

    Ding-Tao Wu, Ren-You Gan & Liang Zou

  3. Research Center for Plants and Human Health, Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu, 610213, China

    Fen Li & Ren-You Gan

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  1. Kang-Lin Feng
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Contributions

K-LF: data curation; formal analysis; investigation; methodology; writing-original draft. LH: data curation; validation; formal analysis; investigation; writing-original draft. D-TW: conceptualization; funding acquisition; data curation; formal analysis; supervision; writing-review and editing. FL: validation; investigation. R-YG: resources; formal analysis. WQ: formal analysis; supervision; writing-review and editing; LZ: conceptualization; formal analysis; supervision; writing-review and editing.

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Correspondence to Ding-Tao Wu, Wen Qin or Liang Zou.

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Feng, KL., Huang, L., Wu, DT. et al. Physicochemical properties and in vitro bioactivities of polysaccharides from lotus leaves extracted by different techniques and solvents. Food Measure 16, 1583–1594 (2022). https://doi.org/10.1007/s11694-021-01256-3

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