Skip to main content

Advertisement

SpringerLink
Log in

Effects of dehydration and extraction techniques on the physicochemical properties and antioxidant activities of Oudemansiella radicata polysaccharides

  • Original Paper
  • Published:
Journal of Food Measurement and Characterization Aims and scope Submit manuscript

Abstract

Oudemansiella radicata is a precious mushroom widely cultivated in China with mature cultivation techniques. The extraction efficiency and biological activity of polysaccharides are crucial for the utilization of O. radicata. In this study, the effects of dehydration (hot air drying and freeze-drying) and extraction methods (heat-reflux extraction, ultrasound-assisted extraction and maceration extraction) on the physicochemical properties and antioxidant activities of Oudermansiella radicata polysaccharides (ORPs) were investigated. Six polysaccharides were obtained, and experimental results indicated that physicochemical and antioxidant properties of ORPs were different from each other after the treatment of various dehydration and extraction techniques. Based on correlation analysis, the antioxidant activities of the ORPs could be attributed to the uronic acid content, molecular weight, and glucose and mannose contents. The freeze-drying and ultrasound-assisted methods are promising and alternative techniques for obtaining ORPs considering their high yield and notable antioxidant activity.

Graphic abstract

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. X. Yang, Z. Sun, W. Wang, Q. Zhou, G. Shi, F. Wei et al., Developmental toxicity of synthetic phenolic antioxidants to the early life stage of Zebrafish. Sci. Total Environ. 643, 559–568 (2018)

    Article  CAS  PubMed  Google Scholar 

  2. Y. Xue, X. Ding, X. Wu, Z. Xu, Z. Zhang, J. He et al., Optimization of preparation process and antioxidant activity of the chelate of a Hericium erinaceus polysaccharide with zinc. J. Food Meas. Charact. 15, 2039–2048 (2021)

    Article  Google Scholar 

  3. X. Chen, H. Ji, C. Zhang, J. Yu, A. Liu, Structural characterization and antitumor activity of a novel polysaccharide from Grifola frondosa. J. Food Meas. Charact. 14, 272–282 (2020)

    Article  Google Scholar 

  4. J. Yan, L. Wu, Z. Qiao, W. Cai, H. Ma, Effect of different drying methods on the product quality and bioactive polysaccharides of bitter gourd (Momordica charantia L.) slices. Food Chem. 271, 588–596 (2019)

    Article  CAS  PubMed  Google Scholar 

  5. B. Le, K.S. Golokhvase, S.H. Yang, S. Sun, Optimization of microwave-assisted extraction of polysaccharides from Ulva pertusa and evaluation of their antioxidant activity. Antioxidants 8, 129 (2020)

    Article  Google Scholar 

  6. S.B. Kim, S.H. Kim, K.R. Lee, J.O. Shim, M.W. Lee, M.J. Shim, The optimal culture conditions for the mycelia growth of Oudemansiella radicata. Mycobiology 33, 230–234 (2005)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Y. Wang, J. Jia, X. Ren, B. Li, Q. Zhang, Extraction, preliminary characterization and in vitro antioxidant activity of polysaccharides from Oudemansiella radicata mushroom. Int. J. Biol. Macromol. 120, 1760–1769 (2018)

    Article  CAS  PubMed  Google Scholar 

  8. Z. Gao, X. Liu, W. Wang, Q. Yang, Y. Dong, N. Xu et al., Characteristic anti-inflammatory and antioxidative effects on enzymatic- and acidic-hydrolysed mycelium polysaccharides by Oudemansiella radicata on LPS-induced lung injury. Carbohyd. Polym. 204, 142–151 (2018)

    Article  Google Scholar 

  9. Z. Zhang, G. Lv, W. He, L. Shi, H. Pan, L. Fan, Effects of extraction methods on the antioxidant activities of polysaccharides obtained from Flammulina velutipes. Carbohyd. Polym. 98, 1524–1531 (2013)

    Article  CAS  Google Scholar 

  10. M. Dubois, K.A. Gilles, J.K. Hamilton, P.A. Rebers, F. Smith, Colorimetric method for determination of sugars and related substances. Anal. Chem. 28, 350–356 (1956)

    Article  CAS  Google Scholar 

  11. J. Spectors, Refinement of the comassie blue method of protein qualification. A simple and linear spectrophotometric assay of 0.5–50 μg protein. Anal. Biochem. 86, 142–146 (1978)

    Google Scholar 

  12. T. Bitter, H. Muir, A modified uronic acid carbazole reaction. Anal. Biochem. 4, 330–334 (1962)

    Article  CAS  PubMed  Google Scholar 

  13. L. Meng, S. Sun, R. Li, Z. Shen, P. Wang, X.L. Jiang, Antioxidant activity of polysaccharides produced by Hirsutella sp. and relation with their chemical characteristics. Carbohyd. Polym. 117, 452–457 (2015)

    Article  CAS  Google Scholar 

  14. R. Yang, J. Zhang, Q. Tang, Y. Pan, High performance anion exchange chromatography method to determine the monosaccharide composition of polysaccharides. Edible Fungi China 24, 42–44 (2005)

    Google Scholar 

  15. O. Erel, A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin. Biochem. 37, 277–285 (2004)

    Article  CAS  PubMed  Google Scholar 

  16. X. Li, A. Zhou, Y. Han, Anti-oxidation and anti-microorgansim activities of purification polysaccharide from Lygodium japonicum in vitro. Carbohyd. Polym. 66, 34–42 (2006)

    Article  CAS  Google Scholar 

  17. M. Oyaizu, Studies on product of browning reaction prepared form glucose amine. Jpn. J. Nutri. 44, 307–315 (1986)

    Article  CAS  Google Scholar 

  18. P. York, Solid-state properties of powders in the formulation and processing of solid dosage forms. Int. J. Pharm. 14, 1–28 (1983)

    Article  CAS  Google Scholar 

  19. C. Ratti, Hot air and freeze-drying of high-value foods: a review. J. Food Eng. 49, 311–319 (2001)

    Article  Google Scholar 

  20. S. Wu, F. Li, S. Jia, H. Ren, G. Gong, Y. Wang et al., Drying effects on the antioxidant properties of polysaccharides obtained from Agaricus blazei Murrill. Carbohyd. Polym. 103, 414–417 (2014)

    Article  CAS  Google Scholar 

  21. I. Alzorqi, S. Sudheer, T. Lu, S. Manickam, Ultrasonically extracted β-D-glucan from artificially cultivated mushroom, characteristic properties and antioxidant activity. Ultrason. Sonochem. 35, 531–540 (2017)

    Article  CAS  PubMed  Google Scholar 

  22. Y. Miao, Q. Lin, Y. Cao, G. He, D. Qiao, Y. Cao, Extraction of water-soluble polysaccharides (WSPS) from Chinese truffle and its application in frozen yogurt. Carbohyd. Polym. 86, 566–573 (2011)

    Article  CAS  Google Scholar 

  23. J. Wang, H. Guo, J. Zhang, X. Wang, B. Zhao, J. Yao et al., Sulfated modification, characterization and structure-antioxidant relationships of Artemisia sphaerocephala polysaccharides. Carbohyd. Polym. 81, 897–905 (2010)

    Article  CAS  Google Scholar 

  24. S. Shirsath, S. Sonawane, P. Gogate, Intensification of extraction of natural products using ultrasonic irradiations-a review of current status. Chem. Eng. Process. 53, 10–23 (2012)

    Article  CAS  Google Scholar 

  25. L. Ma, H. Chen, W. Zhu, Z. Wang, Effect of different drying methods on physicochemical properties and antioxidant activities of polysaccharides extracted form mushroom Inonotus obliquus. Food Res. Int. 50, 633–640 (2013)

    Article  CAS  Google Scholar 

  26. F. Huang, H. Liu, R. Zhang, L. Dong, L. Liu, Y. Ma et al., Physicochemical properties and prebiotic activities of polysaccharides from longan pulp based on different extraction techniques. Carbohyd. Polym. 206, 344–351 (2019)

    Article  CAS  Google Scholar 

  27. Y. Wang, Z. Yang, X. Wei, Sugar compositions, α-glucosidase inhibitory and amylase inhibitory activities of polysaccharides from leaves and flowers of Camellia sinensis obtained by different extraction methods. Int. J. Biol. Macromol. 47, 534–539 (2010)

    Article  CAS  PubMed  Google Scholar 

  28. A. Abuduwaili, P. Rozi, P. Mutailifu, Y. Gao, R. Nuerxiati, H.A. Aisa et al., Effects of different extraction techniques on physicochemical properties and biological activities of polysaccahrides from Fritillaria pallidiflora schrenk. Process Biochem. 83, 189–197 (2019)

    Article  CAS  Google Scholar 

  29. C. Zhao, X. Li, J. Miao, S. Jing, X. Li, L. Huang, The effect of different extraction techniques on property and bioactivity of polysaccharides from Dioscorea hemsleyi. Int. J. Biol. Macromol. 102, 847–856 (2017)

    Article  CAS  PubMed  Google Scholar 

  30. Q. Kang, S. Chen, S. Li, B. Wang, X. Liu, L. Hao et al., Comparison on characterization and antioxidant activity of polysaccharides from Ganoderma lucidum by ultrasound and conventional extraction. Int. J. Biol. Macromol. 124, 1137–1144 (2019)

    Article  CAS  PubMed  Google Scholar 

  31. J. Gao, T. Zhang, Z. Jin, X. Xu, J. Wang, X. Zha et al., Structural characterization, physicochemical properties and antioxidant activity of polysaccharide from Lilium lancifolium Thunb. Food Chem. 169, 430–438 (2015)

    Article  CAS  PubMed  Google Scholar 

  32. D.W. Merkx, Y. Westphal, E.J.J. Velzen, K.V. Thakoer, N. Roo, J.P.M. Duynhoven, Quantification of food polysaccharide mixtures by 1H NMR. Carbohyd. Polym. 179, 379–385 (2018)

    Article  CAS  Google Scholar 

  33. P. Patra, D. Das, B. Behera, T.K. Maiti, S.S. Islam, Structure elucidation of an immunoenhancing pectic polysaccharide isolated from aqueous extract of pods of green bean (Phaseolus vulgaris L.). Carbohyd. Polym. 87, 2169–2175 (2012)

    Article  CAS  Google Scholar 

  34. S. Chen, H. Shang, J. Yang, Y. Li, H. Wu, Effects of different extraction techniques on physicochemical properties and activities of polysaccharides from comfrey (Symphytum officinale L.) root. Ind. Crop. Prod. 121, 18–25 (2018)

    Article  CAS  Google Scholar 

  35. H. Chen, M. Zhang, Z. Qu, B. Xie, Antioxidant activities of different fractions of polysaccharide conjugates from green tea (Camellia Sinensis). Food Chem. 106, 559–563 (2008)

    Article  CAS  Google Scholar 

  36. Y. Xu, F. Cai, Z. Yu, L. Zhang, X. Li, Y. Yang et al., Optimisation of pressurized water extraction of polysaccharides from blackcurrant and its antioxidant activity. Food Chem. 194, 650–658 (2016)

    Article  CAS  PubMed  Google Scholar 

  37. H. Qi, T. Zhao, Q. Zhang, Z. Li, Z. Zhao, R. Xing, Antioxidant activity of different molecular weight sulfated polysaccharides from Ulva pertusa Kjellm (Chlorophyta). J. Appl. Phycol. 17, 527–534 (2005)

    Article  CAS  Google Scholar 

  38. Z. Zhang, X. Wang, C. Liu, J. Li, The degradation, antioxidant and antimutagenic activity of the mucilage polysaccharide from Dioscorea opposite. Carbohyd. Polym. 150, 227–231 (2016)

    Article  CAS  Google Scholar 

  39. G. Chen, C. Fang, C. Ran, Y. Tan, Q. Yu, J. Kan, Comparison of different extraction methods for polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products. Int. J. Biol. Macromol. 130, 903–914 (2019)

    Article  CAS  PubMed  Google Scholar 

  40. H. Chen, X. Xu, Y. Zhu, Optimization of hydroxyl radical scavenging activity of exo-polysaccharides from Inonotus obliquus in submerged fermentation using response surface methodology. J. Microbiol. Biotech. 20, 835–843 (2010)

    CAS  Google Scholar 

  41. H. Shang, S. Chen, R. Li, H. Zhou, H. Wu, H. Song, Influences of extraction methods on physicochemical characteristics and activities of Astragalus cicer L. polysaccharides. Process Biochem. 73, 220–227 (2018)

    Article  CAS  Google Scholar 

  42. Y. Yan, X. Li, M. Wan, J.P. Chen, S.J. Li, M. Cao, D.Y. Zhang, Effect of extraction methods on property and bioactivity of water-soluble polysaccharides from Amomum villosum. Carbohyd. Polym. 117, 632–635 (2015)

    Article  CAS  Google Scholar 

  43. D. Luo, Structural investigation of a polysaccharide (DMB) purified from Dioscorea nipponica Makino. Carbohyd. Polym. 103, 261–266 (2014)

    Article  CAS  Google Scholar 

Download references

Funding

This research was supported by the Agriculture, Rural areas and Farmers and Six-party Project of Zhejiang Province (2019SNLF015) and New Variety Breeding Project of Science Technology Department of Zhejiang Province, China (2021C02073-7).

Author information

Authors and Affiliations

  1. Institute of Horticulture, Zhejiang Academy of Agricultural Science, Hangzhou, 310021, China

    Zhang Zuofa, Wu Chao, Cai Weiming, Song Tingting & Lv Guoying

Authors
  1. Zhang Zuofa
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Wu Chao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Cai Weiming
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Song Tingting
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Lv Guoying
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

ZZ: Funding acquisition, review and editing. CW: validation. TS: Data curation. WC: Visualization. GL: Project administration and investigation.

Corresponding authors

Correspondence to Zhang Zuofa or Lv Guoying.

Ethics declarations

Conflict of interest

The authors declare that they have no competing interests.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file1 (DOC 232 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zuofa, Z., Chao, W., Weiming, C. et al. Effects of dehydration and extraction techniques on the physicochemical properties and antioxidant activities of Oudemansiella radicata polysaccharides. Food Measure 16, 180–190 (2022). https://doi.org/10.1007/s11694-021-01154-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11694-021-01154-8

Keywords

Search

Navigation