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Unravelling the Biohydrogen Production Potential from a Co-Digestion Process of Banana Processing Wastewater and Synthetic Sewage by Anaerobic Fermentation: Performance Evaluation and Microbial Community Analysis

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Abstract

Biohydrogen (bioH2) and soluble metabolites products (SMPs) obtention from the co-digestion process of banana processing wastewater (BPW) and synthetic sewage (SS) were investigated. The reactor performance was evaluated by BPW addition with different initial concentrations: 2.0, 5.0, and 9.6 g of total carbohydrate (TC)/L (pure BPW) using SS to complete the working volume. The dark fermentation process was carried out in a 1 L batch reactor operated at 37 °C/52 h and pH 7. The composition of gas and liquid samples (TC, VFAs, alcohols, and pH) were analyzed during reactor operation. The highest bioH2 production yield (210.82 ± 32.07 NmL/g TC) and bioH2 production rate (40.93 ± 7.60 NmL/g TC/h) were obtained at an initial substrate concentration of 2.0 g TC/L. These results indicated that the co-digestion improved carbohydrate utilization and induced a more effective metabolic pathway to bioH2 production. At this condition, the main soluble metabolite products were acetate and butyrate, whereupon Clostridiacae was the main family involved in BPW fermentation. The prediction of functional gene expression evidenced a shift in the mechanisms of SMPs and bio H2 obtention as the initial concentration of substrate changes.

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Funding

The financial assistance was provided by São Paulo Research Foundation (FAPESP), Grant No. 2020/15155-3.

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Authors and Affiliations

  1. Institute for Research in Bioenergy, São Paulo State University (UNESP), Rio Claro, SP, 13500-230, Brazil

    Cintia Cristina da Costa Freire, Danieli Fernanda Canaver Marin, Caroline de Freitas, Michel Brienzo & Sandra Imaculada Maintinguer

  2. Municipal Institute of Higher Education, Catanduva, SP, 15.800-970, Brazil

    Raissa Cristina da Silva Mazareli

  3. Institute of Chemistry, São Paulo State University (UNESP), Araraquara, SP, 14800-060, Brazil

    Sandra Imaculada Maintinguer

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  1. Cintia Cristina da Costa Freire
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by CCCF, as well as the written of the first draft of the manuscript. CF performed the High-Performance Liquid Chromatography analysis. DFCM contributed to the experimental outline and discussion. SIM and MB made funding acquisition, advising and discussion. All authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Cintia Cristina da Costa Freire.

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da Costa Freire, C.C., Marin, D.F.C., da Silva Mazareli, R.C. et al. Unravelling the Biohydrogen Production Potential from a Co-Digestion Process of Banana Processing Wastewater and Synthetic Sewage by Anaerobic Fermentation: Performance Evaluation and Microbial Community Analysis. Waste Biomass Valor 15, 1587–1601 (2024). https://doi.org/10.1007/s12649-023-02258-6

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