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Kitchen Waste Oil Convert to Biodiesel via W/O Interface Biocatalysis with Thermomyces Lanuginosus Lipase–PNIPAAm Conjugates

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Abstract

Due to the scarcity of resources, alternatives to non-renewable resources have become more important. Chemical catalysts react violently and the conditions are harsh. Using biocatalysts at the water/oil (W/O) interface remains technically challenging and is rewarding. In this study, the Thermomyces lanuginosus lipase-poly coupling biocatalyst was prepared by the atom transfer radical polymerization “grafting-from” method. The TL–PNIPAAm conjugate was successfully prepared and circular dichroism showed a negligible change between the free enzyme and conjugated enzyme by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transmission electron microscopy. Furthermore, the temperature and pH stability of the conjugate was better than that of free TL. The remaining activity of the TL–PNIPAAm conjugate for 10 h was approximately 20% higher than that of free TL after heat treatment (55 °C) and higher pH stability was obtained in the pH 4–10. The remaining activity of the TL–PNIPAAm conjugates was above 60% after recycling 7 times. In addition, the conversion of biodiesel from kitchen waste oil was 92.6% at 50 °C for 25 h with a conversion efficiency of 0.04 g/mL/h. Thus, a high stability and reusability biocatalyst and stabilizer at the interface of water-in-oil Pickering emulsions was successfully prepared.

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Funding

This work was supported by the National Natural Science Foundation of China (Grant No. 21978121) and the 333 High-level Talent Training Project of Jiangsu Province (Grant No. BRA2019281).

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Authors and Affiliations

  1. Jiangsu Key Laboratory of Sericutural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, 212100, Jiangsu, People’s Republic of China

    Can-Yang Shi, Yan Hu, Ya-Li Chai, Cheng-Kun Wu, Ji-Long Wu, Shuai You & Jun Wang

  2. Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture and Rural Affairs, Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, 212100, Jiangsu, People’s Republic of China

    Jun Wang

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  1. Can-Yang Shi
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Shi, CY., Hu, Y., Chai, YL. et al. Kitchen Waste Oil Convert to Biodiesel via W/O Interface Biocatalysis with Thermomyces Lanuginosus Lipase–PNIPAAm Conjugates. Waste Biomass Valor 13, 3945–3956 (2022). https://doi.org/10.1007/s12649-022-01735-8

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