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Optimization of Cellulase Production by Trichoderma Strains Using Crude Glycerol as a Primary Carbon Source with a 24 Full Factorial Design

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Abstract

This work focuses on the optimization of cellulase production by two Trichoderma strains. A 24 full factorial design was used to evaluate the effects of four factors in the optimization of cellulase production (filter paper assay—FPA): crude glycerol, microcrystalline cellulose, yeast extract and ammonium sulfate. In fermentation with Trichoderma CMIAT 054 strain the largest FPA (138.48 FPU L−1) occurred with 25.0 g L−1 of cellulose, 10.0 g L−1 of crude glycerol, 1.4 g L− 1 of yeast extract and 3.5 g L− 1 of ammonium sulfate in the culture medium. In tests with Trichoderma CMIAT 041 strain the highest FPA (89.35 FPU L−1) occurred with 25.0 g L−1 of cellulose, 20.0 g L−1 of crude glycerol, 0.6 g L−1 of yeast extract and 1.5 g L−1 of ammonium sulfate in the culture medium. ANOVA showed a correlation coefficient of 93 and 88% for Trichoderma CMIAT 054 and CMIAT 041 strains, respectively. Reduced regression models for the cellulase produced by these strains were obtained.

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Acknowledgements

The authors would like to thank the Coordination for the Upgrading of Higher Education Personnel (CAPES, Brazil)—Project AUXPE PNPD 2491/2009. Thanks are also due to the Foundation for Support in Scientific and Technological Development (FUNCAP) of the state of Ceará, Brazil.

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  1. National Institute for Amazonian Research (INPA), Avenue André Araújo, 2936, Aleixo, Manaus, AM, 69067-375, Brazil

    Kally Alves de Sousa

  2. Department of Chemical Engineering, Federal University of Ceará (UFC), Bloco 709, Campus do Pici, Fortaleza, CE, 60455-760, Brazil

    Genilton Silva da Faheina Junior & Diana Cristina Silva de Azevedo

  3. Brazilian Agricultural Research Corporation (EMBRAPA), National Research Center for Tropical Agriculture Industry (CNPAT), Street Doctor Sara Mesquita, 2270, Pici, Fortaleza, CE, 60511-110, Brazil

    Genilton Silva da Faheina Junior & Gustavo Adolfo Saavedra Pinto

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  1. Kally Alves de Sousa
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de Sousa, K.A., da Faheina Junior, G.S., de Azevedo, D.C.S. et al. Optimization of Cellulase Production by Trichoderma Strains Using Crude Glycerol as a Primary Carbon Source with a 24 Full Factorial Design. Waste Biomass Valor 9, 357–367 (2018). https://doi.org/10.1007/s12649-016-9806-8

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