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Lactiplantibacillus plantarum Regulated Intestinal Microbial Community and Cytokines to Inhibit Salmonella typhimurium Infection

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Abstract

Lactic acid bacteria (LAB) are recognized as food-grade safe microorganisms and have many beneficial effects. LAB could maintain the host intestinal homeostasis and regulate intestinal microbial community to exert antibacterial effects. In this study, Lactiplantibacillus plantarum (L. plantarum, Lp01) strain isolated from pig intestine was orally administered to C57BL/6 mice, and mice were then infected with Salmonella typhimurium (ATCC14028). The protective effects of L. plantarum were evaluated by monitoring body weight loss, survival rates, bacterial loads in tissue, colon histopathology analysis, and cytokine secretion. 16S rRNA gene sequencing was also utilized to detect the dynamics of the blind gut microbial community in mice. We found that L. plantarum could significantly reduce the body weight loss and improve the survival rates. The survival rate in the L. P-Sty group was up to 67.5%, which was much higher than that in the STY group (25%). Counting of bacterial loads displayed that the colony-forming unit (CFU) of S. typhimurium in the spleen (p < 0.05) and the liver (p < 0.05) from L. P-Sty group both decreased, compared with STY group. Intestinal histopathology showed that it alleviated the intestinal injury caused by Salmonella, inhibited the secretion of pro-inflammatory cytokines, and promoted anti-inflammatory cytokines (p < 0. 01). In addition, L. plantarum also significantly ameliorated the intestinal gut microbiome disturbance caused by Salmonella. It displayed an obvious increase of beneficial bacteria including Lactobacillus and Bacteroidetes and reduction of pathogenic bacteria like Proteobacteria. In conclusion, L. plantarum could regulate microbial community to inhibit Salmonella typhimurium infection.

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Funding

This work was supported by the Jilin Province Science and Technology Project (20200201109JC) and the National Natural Science Foundation of China (31302061).

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  1. Rui-Han Liu and An-Qi Sun have contributed equally to this work.

Authors and Affiliations

  1. College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, China

    Rui-Han Liu, An-Qi Sun, Ye Liao, Zheng-Xu Tang, Shi-Han Zhang, Xin Shan & Jing-Tao Hu

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  1. Rui-Han Liu
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Contributions

Jingtao Hu was corresponding authors to design the experiments. Rui-Han Liu and Ye Liao completed the experiments and wrote the paper. An-Qi Sun analyzed the data. All authors reviewed the results and approved the manuscript.

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Correspondence to Jing-Tao Hu.

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Liu, RH., Sun, AQ., Liao, Y. et al. Lactiplantibacillus plantarum Regulated Intestinal Microbial Community and Cytokines to Inhibit Salmonella typhimurium Infection. Probiotics & Antimicro. Prot. 15, 1355–1370 (2023). https://doi.org/10.1007/s12602-022-09987-5

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