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Effects of Lactic Acid Bacteria Isolated from Equine on Salmonella-Infected Gut Mouse Model

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Abstract

The aim of this study was to evaluate the antibacterial potential of lactic acid bacteria (Weissella confuse, Pediococcus acidilactici, and Ligilactobacillus equi) isolated from healthy equine in Wuhan against Salmonella Typhimurium CVCC542-induced mice model on intestinal microflora. In previous studies, these isolated strains showed good probiotic potentials in vitro. In this study, fifty healthy mice were randomly divided into five groups, the blank control group, the control group, the Pediococcus acidilactici group (1 × 108 CFU/day), the Ligilactobacillus equi group (1 × 108 CFU/day), and the Weissella confuse group (1 × 108 CFU/day). The body weight in control group and Weissella confuse group showed significant decreased (P < 0.05, P < 0.01), while Pediococcus acidilactici group and Ligilactobacillus equi group showed good recovering after treatments. The lowest diarrhea rate was shown in Ligilactobacillus equi group after treatment. In histopathology, Ligilactobacillus equi group showed the least structural damage in duodenum, and all probiotic treatment groups showed less damage in cecum. The sequence data and optical transform unit showed that Pediococcus acidilactici group and Ligilactobacillus equi group had higher number than control group, while the diversity data showed that the control group and Weissella confuse group had lower diversity in cecum. Microbial community analysis showed increased abundance of Firmicutes, Bacteroidetes, uncultured_bacterium_f_Muribaculaceae, and Lactobacillus in treatment groups, while potential microbes that can induce intestinal diseases such as Verrucomicrobia, Akkermansia, and Lachnospiraceae_NK4A136_group decreased in the treatment groups. In conclusion, lactic acid bacteria isolated from the healthy horses could alleviate the infection of Salmonella and regulate intestinal flora.

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Funding

The study was supported by the Fundamental Research Funds for the Central Universities, China (2662017QD015).

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  1. College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China

    Lulu Pei, Juanjuan Liu, Zonghao Huang, ·Mudassar Iqbal & Yaoqin Shen

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LL Pei and YQ Shen proposed and designed the research idea; JJ Liu and ZH Huang contributed sample collection and analysis tools; LL Pei wrote the manuscript; M Iqbal checked the manuscript; YQ Shen checked the manuscript and approved the work done in the study.

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Correspondence to Yaoqin Shen.

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Pei, L., Liu, J., Huang, Z. et al. Effects of Lactic Acid Bacteria Isolated from Equine on Salmonella-Infected Gut Mouse Model. Probiotics & Antimicro. Prot. 15, 469–478 (2023). https://doi.org/10.1007/s12602-021-09841-0

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