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Determination of thermal tolerance in rainbow trout Oncorhynchus mykiss based on effective time, and its reproducibility for a large number of fish

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Abstract

Thermally selected male rainbow trout selectively bred at high temperatures were crossed with females of the Nikko strain with normal thermal tolerance. The F2 pedigrees, comprising 1641 individuals, were divided into 26 batches. The fish were acclimated to 17 °C, then their thermal tolerance determined by measuring the effective time (ET) required for them to lose equilibrium at an approximate upper lethal temperature (28 °C). The fish were categorized into 0- to < 20-, 20- to < 40-, 40- to < 60-, and ≤ 60-min ET groups. The individuals that survived the first treatment were subjected to a second experiment to determine the ET again for their respective groups. High reproducibility, i.e., 100%, for the 0- to < 20-min ET group was observed in the third experiment for individuals grouped into this ET category in the second experiment; 85.5% of individuals classified into the ≤ 60-min ET in the first experiment were recovered in the second experiment. Thus, our findings demonstrate that ET is a useful indicator for discriminating phenotypes according to their thermal tolerance.

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Acknowledgements

This study was supported in part by a grant from the Ministry of Agriculture, Forestry, and Fisheries of Japan. We thank Dr. Tsuchida of the Marine Ecology Research Institute Central Laboratory for his invaluable advice. The authors declare no conflict of interest. We would like to thank Editage (www.editage.com) for the English language editing of the submitted manuscript.

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Authors and Affiliations

  1. Aquaculture Research Institute, Shingu Station, Kindai University, 1330 Takata, Shingu, Wakayama, 647-1101, Japan

    Toshinao Ineno

  2. Miyazaki Prefectural Fisheries Research Institute Freshwater Branch, Kobayashi, Miyazaki, 886-0005, Japan

    Toshinao Ineno, Kazuya Yamada, Koichi Tamaki & Ryusuke Kodama

  3. ACTmed Company Limited, Nihonbashi-Honcho 3-11-5, Chuo, Tokyo, 103-0023, Japan

    Engkong Tan

  4. Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo, 113-8657, Japan

    Engkong Tan, Shigeharu Kinoshita, Shuichi Asakawa & Shugo Watabe

  5. Research Center for Freshwater Fisheries, National Research Institute of Fisheries Science, Japan Fisheries Research and Education Agency, Tochigi, 321-1661, Japan

    Koji Muto, Takashi Yada & Shoji Kitamura

  6. School of Marine Biosciences, Kitasato University, Sagamihara, Kanagawa, 252-0373, Japan

    Shugo Watabe

Authors
  1. Toshinao Ineno
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  2. Kazuya Yamada
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  3. Koichi Tamaki
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  4. Ryusuke Kodama
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  5. Engkong Tan
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  6. Shigeharu Kinoshita
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  7. Koji Muto
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  8. Takashi Yada
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  9. Shoji Kitamura
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  10. Shuichi Asakawa
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  11. Shugo Watabe
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Correspondence to Toshinao Ineno.

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Ineno, T., Yamada, K., Tamaki, K. et al. Determination of thermal tolerance in rainbow trout Oncorhynchus mykiss based on effective time, and its reproducibility for a large number of fish. Fish Sci 86, 767–774 (2020). https://doi.org/10.1007/s12562-020-01447-9

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  • DOI: https://doi.org/10.1007/s12562-020-01447-9

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