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Overexpression of V-ATPase c Subunit Gene (VHA-c) from Antarctic Notothenioid Fish Improves Tolerance of Transgenic Arabidopsis to Low Temperature

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Abstract

To understand the possible role of vacuolar H+-ATPase c subunit in resistance to low temperature, the VHA-c gene was isolated from Antarctic notothenioid fishes (Dissostichus mawsoni), and two overexpression plants were constructed and obtained from Arabidopsis: VHA-c8 and VHA-c11. The physiological responses of VHA-c overexpression plants and wild-type Arabidopsis to low temperature were studied at 4 °C. Arabidopsis with overexpression of VHA-c showed stronger low-temperature tolerance than wild type. The membrane damage indexes (malondialdehyde and membrane leakage rate) of wild-type plants were significantly higher than those of overexpressing lines VHA-c8 and VHA-c11, and wild-type plants are more susceptible to photoinhibition than VHA-c8 and VHA-c11 at low temperature. In addition, the content of various osmoregulatory substances in wild-type plants, including proline, soluble protein and soluble sugar, was significantly lower than that of VHA-c8 and VHA-c11. However, the ATP content of VHA-c8 and VHA-c11 was significantly higher than that of wild-type plants. The change of ATP content is consistent with the change of osmoregulatory substances content, and is opposite to the change of membrane damage indexes. These results suggest that VHA-c gene may provide energy for the synthesis of osmoregulatory substances by promoting the increase of ATP synthesis, so as to improve the cold resistance of Arabidopsis. In addition, the increased ATP can also be used as a compensatory strategy under low temperature.

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Funding

This research was funded by the National Natural Science Foundation of China (31870374) and the National Special Project for Genetic Improving of Agricultural Species (2009ZX08009003B).

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  1. Wei Lin and Zhengchao Yu have contributed equally to this work.

Authors and Affiliations

  1. Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou, 510631, China

    Wei Lin, Wei He & Changlian Peng

  2. School of Life Sciences and Food Engineering, Hanshan Normal University, Chaozhou, 521041, China

    Zhengchao Yu

  3. College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, 524088, China

    Tingqin Wang

  4. Internal Joint Research Center for Marine Biosciences (Ministry of Science and Technology), College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China

    Liangbiao Chen

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Conceptualization, TQW; Data curation, ZCY; Investigation, WH; Supervision, LBC and CLP; Writing—original draft, WL, and ZCY.

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Correspondence to Changlian Peng.

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Lin, W., Yu, Z., Wang, T. et al. Overexpression of V-ATPase c Subunit Gene (VHA-c) from Antarctic Notothenioid Fish Improves Tolerance of Transgenic Arabidopsis to Low Temperature. J. Plant Biol. 66, 567–578 (2023). https://doi.org/10.1007/s12374-023-09407-3

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