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Genetic Structure Analysis in Sugarcane (Saccharum spp.) Using Target Region Amplification Polymorphism (TRAP) Markers Based on Sugar- and Lignin-Related Genes and Potential Application in Core Collection Development

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Abstract

Sugarcane is a main bioenergy crop and is highlighted worldwide in sugar, ethanol, and biomass production. Sugar and lignin contents are important quality traits for traditional and energy cane cultivar development, respectively. In the present study, genetic variability of a broad group of sugarcane basic germplasm accessions encompassing wild relatives and traditional and modern cultivars was assessed using target region amplification polymorphism (TRAP) markers derived from candidate genes involved in sugar and lignin metabolism. In total, 823 polymorphic markers (483 for sugar and 340 for lignin metabolism genes) were amplified; the average polymorphism information content values were highest for sugarcane wild relatives followed by traditional cultivars and modern cultivars. Genetic variability of the 96 genotypes captured by TRAP candidate genes for sugar and lignin metabolism was structured into two and three subpopulations, respectively. Based on the membership proportion (Q), modern cultivars inherited variability for genes involved in sugar metabolism from both S. officinarum and S. spontaneum. The genetic differentiation index based on sugar and lignin metabolism genes suggests moderate genetic differentiation among wild relatives, traditional cultivars, and modern cultivars. A core collection was established for sugar and lignin TRAP markers. Values for average genetic distance for the core collection based on sugar (0.761) and lignin (0.804) TRAP-derived markers were higher than those observed for all accessions, indicating that the core collections retained the most divergent accessions.

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Acknowledgements

The authors are grateful to the Fundacão de Amparo à Pesquisa do Estado de São Paulo (FAPESP Grant: 2013/22500-5).

Funding

This study was funded by FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo (2013/22500-5).

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Authors and Affiliations

  1. Programa de Pós-Graduação em Produção Vegetal, Universidade Estadual de Santa Cruz – UESC, Ilhéus, BA, Brazil

    Carlos Augusto Dias Kanthack Junior

  2. Estação de Hibridação de Cana do Programa Cana, Instituto Agronômico de Campinas, Uruçuca, BA, Brazil

    Carlos Augusto Dias Kanthack Junior

  3. Centro de Cana, Instituto Agronômico de Campinas, Anel Viário Contorno Sul Km 321, CP 206, Ribeirão Preto, SP, 14001-970, Brazil

    João Ricardo Vieira Manechini, Juliana Borges da Costa, Thais Monteiro Favero & Luciana Rossini Pinto

  4. Departamento de Produção Vegetal, Universidade Estadual Paulista, Campus de Jaboticabal, Jaboticabal, SP, Brazil

    João Ricardo Vieira Manechini & Ana Christina Rossini Pinto

  5. Centro de Biotecnologia e Genética, Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Ilhéus, 45662-900, Brazil

    Ronan Xavier Corrêa

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  1. Carlos Augusto Dias Kanthack Junior
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Contributions

CADKJ contributed to data analysis and manuscript writing. JRVM helped in data organization and analysis. JBC and TMF worked for DNA extraction and genotyping. ACRP contributed to manuscript writing and editing. LRP and RXC helped in design and conceptualization of the experiment and research funding. All authors read and approved the final manuscript.

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Correspondence to Luciana Rossini Pinto.

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Junior, C.A.D.K., Manechini, J.R.V., Corrêa, R.X. et al. Genetic Structure Analysis in Sugarcane (Saccharum spp.) Using Target Region Amplification Polymorphism (TRAP) Markers Based on Sugar- and Lignin-Related Genes and Potential Application in Core Collection Development. Sugar Tech 22, 641–654 (2020). https://doi.org/10.1007/s12355-019-00791-0

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