Abstract
Plumbago rosea L. (Plumbaginaceae), is a medicinal shrub commercially exploited for its naphthoquinone principle, plumbagin, extracted from the roots especially for treating skin disorders. As the plant is exploited from the wild without being replenished, conservation of the species becomes inevitable. Synthetic seeds would provide for effective conservation, germplasm exchange and distribution of this species. A reliable protocol for synthetic seed production in Plumbago rosea has been developed encapsulating the axillary buds. The axillary buds from P. rosea cultures established and multiplied using the nodal explants in Murashige and Skoog (MS) medium supplemented with Benzyl Adenine (BA) 1.5 mg/L and Indole 3-Acetic acid 1.0 mg/L, were used for synseed production. The plantlet conversion efficiency was the highest in synthetic seeds developed with sodium alginate 2.5% in modified MS with 0.4 M sucrose and CaCl2 100 mM. This combination gave the earliest bud initiation (9.19 ± 0.39 days) and maximum number of shoots per explant (2.31 ± 0.16 shoots). Microshoots from the culture, when inoculated on to MS medium supplemented with Naphthalene Acetic Acid 1.0 mg/L gave the best rooting response with 10.67 ± 0.94 roots per plant and 5.42 ± 0.29 cm root length. This is the first report of synthetic seed production in P. rosea using axillary buds as explant.
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Abbreviations
- BA:
-
N6-Benzylaminopurine
- Kn:
-
Kinetin (6-furfurylaminopurine)
- IBA:
-
Indole 3-butyric acid
- IAA:
-
Indole 3-acetic acid
- NAA:
-
α-Naphthaleneacetic acid
- MS:
-
Murashige and Skoog
- SA:
-
Sodium alginate
References
Agastian P, Lincy W, Ignacimuthu S (2006) In vitro propagation of Justicia gendarussa Burm. f.—a medicinal plant. Indian J Biotech 5:246–248
Ahmad N, Anis M (2010) Direct plant regeneration from encapsulated nodal segments of Vitex negundo. Biol Plant 54:748–752. https://doi.org/10.1007/s10535-010-0134-8
Ai PF, Lu LP, Song JJ (2012) Cryopreservation of in vitro-grown shoot-tips of Rabdosia rubescens by encapsulation–dehydration and evaluation of their genetic stability. Plant Cell Tissue Organ Cult 108:381–387. https://doi.org/10.1007/s11240-011-0049-x
Anuf AR, Ramachandran R, Krishnasamy RPS, Gandhi S, Periyasamy S (2014) Antiproliferative effects of Plumbago rosea and its purified constituent plumbagin on SK-MEL 28 melanoma cell lines. Pharmacog Res 6:312–319. https://doi.org/10.4103/0974-8490.138280
Asthana P, Jaiswal VS, Jaiswal U (2011) Micropropagation of Sapindus trifoliatus L. and assessment of genetic fidelity of micropropagated plants using RAPD analysis. Acta Physiol Pl 33:1821–1829
Benelli C (2016) Encapsulation of shoot tips and nodal segments for in vitro storage of “Kober 5BB” grapevine rootstock. Horticulturae 2:1–8. https://doi.org/10.3390/horticulturae2030010
Bhanuprakash K, Umesha (2015) Seed Biology and Technology. In: Bahadur B, Venkat Rajam M, Sahijram L, Krishnamurthy K (eds) Plant Biology and Biotechnology. Springer, New Delhi, pp 468–498
Chuakul W, Soonthornchareonnon N, Saralamp P (1999) Plumbago indica L. In: de Padua LS, Bunyapraphatsara N, Lemmens RHMJ (eds) Plant resources of South-East Asia No. 12(1): medicinal and poisonous plants 1. Backhuys Publisher, Leiden, pp 411–412
Duhoky MMS, Rasheed KA (2010) Effect of different concentrations of kinetin and NAA on micropropagation of Gardenia jasminoides. J Zankoy Sulaimani 13:103–120
Gantait S, Kundu S, Ali N, Sahu NC (2015) Synthetic seed production of medicinal plants: a review on influence of explants, encapsulation agent and matrix. Acta Physiol Plant 37:98. https://doi.org/10.1007/s11738-015-1847-2
Gopalakrishnan M, Janarthananm B, Lakshmi SG, Sekar T (2009) Plant regeneration from leaf explants of Plumbago rosea L. Plant Tissue Cult Biotechnol 19:79–87. https://doi.org/10.3329/ptcb.v19i1.4989
Jayasheelan M, Rao M (1998) In vitro propagation of Cardiospermum halicacabum. In: National conference on recent trends spices medicinal plants research, 2–4 April 1998. Abstract. Bose Institute, Calcutta, p 19
Kaewbumrung S, Panichayupakaranant P (2014) Antibacterial activity of plumbagin derivative-rich Plumbago indica root extractsand chemical stability. Nat Prod Res 28:835–837. https://doi.org/10.1080/14786419.2013.879585
Krishna H, Singh SK, Minakshi G, Patel VB, Khawale RN, Deshmukh PS, Jindal PC (2006) Arbuscularmycorrhizal fungi alleviate transplantation shock in micropropagated grapevine (Vitis vinifera L.). J Hortic Sci Biotechnol 81:259–263. https://doi.org/10.1080/14620316.2006.11512059
Krishna H, Alizadeh M, Singh D et al (2016) Somaclonal variations and their applications in horticultural crops improvement. 3. Biotech 6(1):54. https://doi.org/10.1007/s13205-016-0389-7
Maqsood M, Mujib A, Siddiqui ZH (2012) Synthetic Seed development and conversion to plantlet in Catharanthus roseus (L.) G. Don. Biotechnology 11:37–43. https://doi.org/10.3923/biotech.2012.37.43
Mishra J, Singh M, Palni LMS, Nandi SK (2011) Assessment of genetic fidelity of encapsulated micro shoots of Picrorhiza kurrooa. Plant Cell Tissue Organ Cult 104:181–186. https://doi.org/10.1007/s11240-010-9816-3
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Nair DS, Reghunath BR (2007) Effective use of encapsulation–dehydration technique in cryopreserving somatic embryos of butterfly pea (Clitoria ternatea L.). J Herbs Spices Med Plants 13:83–95. https://doi.org/10.1300/J044v13n03_07
Nair DS, Reghunath BR (2009) Cryoconservation and regeneration of axillary shoot meristems of Indigofera tinctoria (L.) by encapsulation–dehydration technique. In Vitro Cell Dev Biol Plant 45:565–573. https://doi.org/10.1007/s11627-009-9244-4
Oh T, Yun J, Park E, Kim Y, Lee Y, Lim J (2017) Plumbagin suppresses α-MSH-induced melanogenesis in B16F10 mouse melanoma cells by inhibiting tyrosinase activity. Int J Mol Sci 18(2):320. https://doi.org/10.3390/ijms18020320
Padro MDA, Frattarelli A, Sgueglia A, Condello E, Damiano C, Caboni E (2012) Cryopreservation of white mulberry (Morus alba L.) by encapsulation–dehydration and vitrification. Plant Cell Tissue Organ Cult 108:167–172. https://doi.org/10.1007/s11240-011-0017-5
Rai MK, Aathana P, Singh SK, Jaiswal VS, Jaiswal U (2009) The encapsulation technology in fruit plants—a review. Biotechnol Adv 27:671–679. https://doi.org/10.1016/j.biotechadv.2009.04.025
Ramesh M, Marx R, Mathan G, Pandian SK (2009) Effect of bavistin on in vitro plant conversion from encapsulated uninodal microcuttings of micropropagated Bacopa monnieri (L.)—an ayurvedic herb. J Environ Biol 30:441–444
Ray A, Bhattacharya S (2008) Storage and plant regeneration from encapsulated shoot tips of Rauvolfia serpentina—an effective way of conservation and mass propagation. S Afr J Bot 74:776–779. https://doi.org/10.1016/j.sajb.2008.06.002
Saiprasad GVS (2001) Artificial seeds and their applications. Resonance 6:39–47. https://doi.org/10.1007/BF02839082
Satheeshkumar K, Bhavananadan KV (1988) Micropropagtion of Plumbago rosea L. Plant Cell Tissue Organ Cult 15:275–278
Sharma S (2017) Plumbago indica plant medicinal uses and common name. www.medicinalplantsanduses.com. https://www.medicinalplantsanduses.com/plumbago-indica-plant-medicinal-uses. Accessed 22 Feb 2018
Singh AK, Varshney R, Sharma M, Agarwal SS, Bansai KC (2006) Regeneration of plants from alginate encapsulated shoot tips of Withania somnifera (L.) Dunal. A medicinally important plant species. J Plant Physiol 163:220–223. https://doi.org/10.1016/j.jplph.2005.06.013
Srivastava V, Khan SA, Banerjee S (2009) An evaluation of genetic fidelity of encapsulated microshoots of the medicinal plant: Cineraria maritima following six months of storage. Plant Cell Tiss Org Cult 99:193–198. https://doi.org/10.1007/s11240-009-9593-z
Turker AU, Guner B (2013) Efficient plant regeneration of yellow loosestrife (Lysimachia vulgaris L.), a medicinal plant. Acta Biol Hung 64:218–230
Verma SK, Raj MK, Asthana P, Jaiswal VS, Jaiswal U (2010) In vitro plantlets from alginate- encapsulated shoot tips of Solanum nigrum L. Sci Hortic 124:517–521. https://doi.org/10.1016/j.scienta.2010.02.002
Xue YL, Meng XQ, Ma LJ, Yuan Z (2016) Plumbagin exhibits an anti-proliferative effect in human osteosarcoma cells by downregulating FHL2 and interfering with Wnt/β-catenin signalling. Oncol Lett 12(2):1095–1100. https://doi.org/10.3892/ol.2016.4725
Yelne MB, Borkar GB, Shrama PC (1997) In vitro propagation of Brahmi-Bacopa monnieri (L.) Pennell. Bull Medicoethnobot Res 18:145–150
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The financial assistance and facilities provided for the research work by Kerala Agricultural University, India is greatly acknowledged.
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Prakash, A.V., Nair, D.S., Alex, S. et al. Calcium alginate encapsulated synthetic seed production in Plumbago rosea L. for germplasm exchange and distribution. Physiol Mol Biol Plants 24, 963–971 (2018). https://doi.org/10.1007/s12298-018-0559-7
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DOI: https://doi.org/10.1007/s12298-018-0559-7