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Physiological traits associated with heat tolerance in bread wheat (Triticum aestivum L.)

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Abstract

Field experiments for evaluating heat tolerance-related physiological traits were conducted for two consecutive years using a mapping population of recombinant inbred lines (RILs) from the cross RAJ4014/WH730. Chlorophyll content (Chl) and chlorophyll fluorescence (CFL) were recorded under timely sown (TS) and late sown (LS) conditions. Late sowing exposes the terminal stage of plants to high temperature stress. Pooled analysis showed that CFL and Chl differed significantly under TS and LS conditions. The mean value of CFL (Fv/Fm) and Chl under both timely and late sown conditions were used as physiological traits for association with markers. Regression analysis revealed significant association of microsatellite markers viz., Xpsp3094 and Xgwm131 with coefficients of determination (R 2) values for CFL (Fv/Fm) and Chl as 12 and 8 %, respectively. The correlation between thousand grain weight (TGW) with Chl and CFL were 14 and 7 % and correlation between grain wt./spike with Chl and CFL were 15 and 8 %, respectively. The genotypes showing tolerance to terminal heat stress as manifested by low heat susceptibility index (HSI = 0.43) for thousand grain weight, were also found having very low Chl, HSI (−0.52). These results suggest that these physiological traits may be used as a secondary character for screening heat-tolerant genotypes.

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Abbreviations

Chl:

Chlorophyll content

CFL:

Chlorophyll fluorescence

TGW:

Thousand grain weight

SSR:

Simple sequence repeat

RIL:

Recombinant inbred lines

Fv/Fm:

Variable fluorescence/maximal fluorescence

HSI:

Heat susceptibility index

QTLs:

Quantitative trait loci

GFD:

Grain filling duration

DPA:

Days to post anthesis

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Acknowledgments

The authors acknowledge the ICAR for financial support for carrying out the work under NPTC: Functional Genomics in wheat project.

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Authors and Affiliations

  1. Directorate of Wheat Research, Karnal, 132001, Haryana, India

    Girish Chandra Pandey, H. M. Mamrutha, Ratan Tiwari, Sindhu Sareen, Vinod Tiwari & Indu Sharma

  2. Department of BT and BI, Jaypee University of Information Technology, Solan, 173234, Himachal Pradesh, India

    Shrutkirti Bhatia

  3. Chaudhary Devi Lal University, Sirsa, 125055, Haryana, India

    Priyanka Siwach

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  1. Girish Chandra Pandey
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  2. H. M. Mamrutha
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  3. Ratan Tiwari
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  4. Sindhu Sareen
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  5. Shrutkirti Bhatia
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  6. Priyanka Siwach
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  7. Vinod Tiwari
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  8. Indu Sharma
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Correspondence to Ratan Tiwari.

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Pandey, G.C., Mamrutha, H.M., Tiwari, R. et al. Physiological traits associated with heat tolerance in bread wheat (Triticum aestivum L.). Physiol Mol Biol Plants 21, 93–99 (2015). https://doi.org/10.1007/s12298-014-0267-x

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  • DOI: https://doi.org/10.1007/s12298-014-0267-x

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