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Screening and identification of Aspergillus activity against Xanthomonas oryzae pv. oryzae and analysis of antimicrobial components

  • Microbial Physiology and Biochemistry
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Abstract

To screen for Aspergillus activity against Xanthomonas oryzae pv. oryzae and analyse the antimicrobial components involved, 60 Aspergillus spp. were isolated and purified from fruits, soil and other habitats. As-75, an Aspergillus strain that can antagonize Xanthomonas oryzae pv. oryzae, was identified based on the zone of inhibition formed during co-culture. According to morphological, ITS rDNA gene sequencing and phylogenetic tree results, the strain showed close homology to Aspergillus sclerotiorum. The biochemical characterization tests showed that the fermentation broth of strain As-75 exhibited a high capacity for environmental adaptation. The results of the antimicrobial spectrum experiments demonstrated that As-75 exhibited fairly strong antagonistic activity against five plant pathogenic fungi and six plant pathogenic bacteria in vitro. The fermentation broth of strain As-75 displayed maximum stability under fluorescent illumination at temperatures below 60°C at pH 6.5. A substance with antagonistic activity was obtained from strain As-75 via fractional extraction, silica gel column chromatography and thin-layer chromatography. Through mass spectrometry, nuclear magnetic resonance and electrospray ionization mass spectrometry (ESI-MS) analyses, the target compound was identified as (2Z)-2-butenedioic acid-2-(1-methylethenyl)-4-methyl ester; its molecular weight of 170.06 daltons and formula of C8H10O4 identify it as a novel compound. Trials of the preventative and curative effects demonstrated that compound S1 exhibited a better control efficiency than the control against rice bacterial blight. Additionally, the M1 processing method was better, and the efficiency of compound S1 in preventing rice bacterial blight in six rice varieties, TN1, IR24, ZF802, Zhonghua 11, Wuyunjing 21, and Nipponbare, was 78.3%, 77.5%, 74.2%, 75.3%, 70.9%, and 72.1%, respectively.

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Acknowledgements

This research was supported by the National Natural Science Foundation of China (Grant 31570013) and was supported by the Zhejiang Provincial Basic Public Welfare Research Project of China (Grant GN18C010004).

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Authors and Affiliations

  1. College of Chemistry and Life Science, Zhejiang Normal University, Zhejiang, 321004, P. R. China

    Bei Jiang, Zhiying Wang, Chuxuan Xu, Weijia Liu & Donghua Jiang

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  1. Bei Jiang
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  2. Zhiying Wang
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  3. Chuxuan Xu
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  4. Weijia Liu
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  5. Donghua Jiang
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Correspondence to Donghua Jiang.

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Jiang, B., Wang, Z., Xu, C. et al. Screening and identification of Aspergillus activity against Xanthomonas oryzae pv. oryzae and analysis of antimicrobial components. J Microbiol. 57, 597–605 (2019). https://doi.org/10.1007/s12275-019-8330-5

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  • DOI: https://doi.org/10.1007/s12275-019-8330-5

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