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In vitro antimicrobial activity of the leaf essential oil of Spiraea alpina Pall.

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Abstract

The qualitative and quantitive determination of chemical components of leaf essential oil of Spiraea alpina Pall. with Microwave-assisted Hydrodistillation is carried out by gas chromatography-mass spectrometry. About 69 compounds have been identified from the leaf oil, accounting for 79.39% of the total. The in vitro antifungal activity of S. alpina essential oil was studied against eight test phytopathogenic bacteria and fungi namely Xanthomonas oryzae pv. oryzae, Xanthomonas campestris pv. citri, Ralstonia solanacearum, Erwinia carotovora subsp. carotovora and Rhizoctonia solani, Fusarium graminerum, Pyricularia oryzea, Exserohilum turcicum by the agar Well Diffusion Method and Poisoned Food Technique, respectively. In the case, R. solanacearum was found to be sensitive to S. alpina oil at a concentration of 10 μl·well−1 and the inhibition zone diameter was found to be 10.7 mm. Concentration- and time-dependent fungitoxicity was recorded from 125 to 1,000 μg·ml−1 concentration. About 125 μg·ml−1 of leaf oil solution partially inhibited the mycelial growth of R. solani to the same extent as 50 μg·ml−1 of miconazole. The oil also affected the mycelial growth of F. graminerum and E. turcicum in a dose-dependent manner but had a weak effect on the growth of P. oryzea.

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Abbreviations

ANOVA:

Analysis of variance

DMSO:

Dimethyl sulfoxide

GC–MS:

Gas chromatography combined with mass spectrometry

IPDM:

Integrated pest and disease management

LSD:

Least significant difference

MAHD:

Microwave-assisted hydrodistillation

MGI:

Mycelial growth inhibition

PDA:

Potato dextrose agar

PTFE:

Polytetrafluoroethylene

RI:

Retention indices

SI:

Similarity index

References

  • Al-Burtamani SKS, Fatope MO, Marwah RG, Onifade AK, Al-Saidi SH (2005) Chemical composition, antibacterial and antifungal activities of the essential oil of Haplophyllum tuberculatum from Oman. J Ethnopharmacol 96:107–112

    Article  CAS  Google Scholar 

  • Bakkali F, Averbeck S, Averbeck D, Idaomar M (2008) Biological effects of essential oils—a review. Food Chem Toxicol 46:446–475

    Article  CAS  Google Scholar 

  • Burt S (2004) Essential oils: their antibacterial properties and potential applications in foods—a review. Int J Food Microbiol 94:223–253

    Article  CAS  Google Scholar 

  • Daferera DJ, Ziogas BN, Polissiou MG (2000) GC–MS analysis of essential oils from some Greek aromatic plants and their fungitoxicity on Penicillium digitatum. J Agric Food Chem 48:2576–2581

    Article  CAS  Google Scholar 

  • Daferera DJ, Ziogas BN, Polissiou MG (2003) The effectiveness of plant essential oils in the growth of Botrytis cinerea, Fusarium sp. and Clavibacter michiganensis subsp. michiganensis. Crop Prot 22:39–44

    Article  CAS  Google Scholar 

  • Delaquis PJ, Stanich K, Girard B, Mazza G (2002) Antimicrobial activity of individual and mixed fractions of dill, cilantro, coriander and eucalyptus essential oils. Int J Food Microbiol 74:101–109

    Article  CAS  Google Scholar 

  • Golmakani MT, Rezaei K (2008) Comparison of microwave-assisted hydrodistillation with the traditional hydrodistillation method in the extraction of essential oils from Thymus vulgaris L. Food Chem 109:925–930

    Article  CAS  Google Scholar 

  • Grover RK, Moore JD (1962) Toximetric studies of fungicides against the brown rot organisms, Sclerotinia fruticola and S. laxa. J Phytopathol 52:876–880

    CAS  Google Scholar 

  • Hao X, Shen Y, Li L, He H (2003) The chemistry and biochemistry of Spiraea japonica complex. Curr Med Chem 10:2253–2263

    Article  CAS  Google Scholar 

  • Hiradatea S, Moritab S, Sugiea H, Fujiia Y, Haradab J (2004) Phytotoxic cis-cinnamoyl glucosides from Spiraea thunbergii. Phytochemistry 65:731–739

    Article  Google Scholar 

  • Janssen AM, Scheffer JJ, Baerheim SA (1987) Antimicrobial activity of essential oils a literature review (1976–1986)—aspects of the test method. Planta Med 53:395–398

    Article  CAS  Google Scholar 

  • Juliani HR Jr, Biurrun F, Koroch AR, Oliva MM, Demo MS, Trippi VS (2002) Chemical constituents and antimicrobial activity of the essential oil of Lantana xenica. Planta Med 68:762–764

    Article  CAS  Google Scholar 

  • Kang Y, Carlson R, Tharpe W, Schell MA (1998) Characterization of genes involved in biosynthesis of a novel antibiotic from Burkholderia cepacia BC11 and their role in biological control of Rhizoctonia solani. Appl Environ Microbiol 64:3939–3947

    CAS  Google Scholar 

  • Lens-Lisbonne C, Cremieux A, Maillard C, Balansard G (1987) Methodes d’evaluation de l’activité antibacterienne des huiles essentielles: application aux essences de thymet de cannelle. J Pharm Belg 42:297–302

    CAS  Google Scholar 

  • Marino M, Bersani C, Comi G (2001) Impedance measurements to study the antimicrobial activity of essential oils from Lamiaceae and Compositae. Int J Food Microbiol 67:187–195

    Article  CAS  Google Scholar 

  • Oliva MM, Demo MS, Lopez AG, Lopez ML, Zygadlo J (2005) Antimicrobial activity and composition of Hyptis mutabilis essential oil. J Herbs Spices Med Plants 11:59–65

    Article  Google Scholar 

  • Roy NK, Dureja P (1998) New ecofriendly pesticides for Integrated Pest Management. Pestic World 3:16–21

    Google Scholar 

  • Yahyazadeh M, Omidbaigi R, Zare R, Taheri T (2008) Effect of some essential oils on mycelial growth of Penicillium digitatum Sacc. World J Microbiol Biotechnol 24:1445–1450

    Article  Google Scholar 

  • Zhou L, Gao F, Teng Y, Hou TP (2008) The bioactivity study of seventeen kinds of poisonous plants from grassland against four pathogenic fungi. J Sichuan Univ (Nat Sci Ed) 45:214–219

    Google Scholar 

Download references

Acknowledgments

The authors are thankful to the Head, College of Life Sciences, College of Chemistry, Pharmaceutical School of Sichuan University, for the elemental and spectral recording, to the Head, Department of Botany, Prof. Hou, for providing the laboratory and microbial facilities, to the Head, Sichuan Agricultural Science Research Institute, Dr. Zhang, for the supply of phytopathogenic fungi. This study was supported by the grants from National Natural Science Foundation of China (NSFC, grant no. 20572076) and National Key Technology R&D Program in the 11th 5-year Plan of China (no. 2006BAE01A01-14).

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Authors and Affiliations

  1. Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, 610064, Chengdu, China

    Yun Teng, Qian Yang & Taiping Hou

  2. Department of Medicinal Chemistry, West China School of Pharmacy, Sichuan University, 610041, Chengdu, China

    Zhiyi Yu, Guoping Zhou & Qiu Sun

  3. Key Laboratory of Green Chemistry and Technology of Ministry of Education, College of Chemistry, Sichuan University, 610064, Chengdu, China

    Hong Jin

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  1. Yun Teng
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  2. Qian Yang
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  4. Guoping Zhou
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  6. Hong Jin
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Correspondence to Taiping Hou.

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Teng, Y., Yang, Q., Yu, Z. et al. In vitro antimicrobial activity of the leaf essential oil of Spiraea alpina Pall.. World J Microbiol Biotechnol 26, 9–14 (2010). https://doi.org/10.1007/s11274-009-0134-z

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  • DOI: https://doi.org/10.1007/s11274-009-0134-z

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