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A quadruplex immunochromatographic assay for the ultrasensitive detection of 11 anesthetics

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Abstract

Anesthetic residues in fish represent a potential risk to human health. Therefore, it is important to develop a sensitive and broad-specific method for the detection of anesthetics. In this study, we developed a colloidal gold-based quadruplex immunochromatographic (Qua-ICS) assay using four highly sensitive monoclonal antibody immunotherapy (mAbs) that simultaneously detected 11 anesthetic residues in fish within 10 min. The colorimetric and cut-off values (COVs) for procaines, eugenols, bupivacaines, and tricaine (TMS) were 0.37–1.1 and 3.3–10, 11–222 and 100–2000, 0.37 and 3.3, and 111 and 10,000 µg/kg, respectively. Quantitative analysis was achieved with a portable strip-reader, and the detection ranges were 0.15–2.6, 6.3–677, 0.13–2.8, and 83–1245 µg/kg for procaines, eugenols, bupivacaines, and tricaine, respectively. Our developed method was reliable and accurate according to the recovery test results and analyses of real samples. Therefore, the strip can be used as an alternative method for the rapid detection of anesthetic residues in fish.

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Acknowledgements

This work is financially supported by the National Key Research and Development Program of China (No. 2022YFF1101002), and Jiangsu Association for Science and Technology Youth Science and Technology Talent Support Project (No. TJ-2021-049).

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Authors and Affiliations

  1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China

    Xianlu Lei, Xinxin Xu, Li Wang, Liqiang Liu, Liguang Xu, Hua Kuang & Chuanlai Xu

  2. International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China

    Xianlu Lei, Xinxin Xu, Li Wang, Liqiang Liu, Liguang Xu, Hua Kuang & Chuanlai Xu

  3. Jiangsu Product Quality Testing and Inspection Institute, Nanjing, 210000, China

    Wei Zhou

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  1. Xianlu Lei
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  2. Xinxin Xu
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  3. Li Wang
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  4. Wei Zhou
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  5. Liqiang Liu
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  6. Liguang Xu
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  7. Hua Kuang
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Correspondence to Xinxin Xu or Hua Kuang.

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Lei, X., Xu, X., Wang, L. et al. A quadruplex immunochromatographic assay for the ultrasensitive detection of 11 anesthetics. Nano Res. 16, 11269–11277 (2023). https://doi.org/10.1007/s12274-023-5768-x

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  • DOI: https://doi.org/10.1007/s12274-023-5768-x

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