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An Aligned Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Scaffold Fixed with Fibronectin to Enhance the Attachment and Growth of Human Endothelial Progenitor Cells

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Abstract

Repair and regeneration of vascular tissue is a crucial current research focus in the fields of biomedical engineering and regenerative medicine. Numerous studies revealed that cells are required to grow on an appropriate extracellular matrix to maintain or enhance functionality. In the present study, various surface modification methods were evaluated to fix fibronectin on the surface of a bio-based and aligned poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) film for vascular tissue engineering. After chemical modification, the properties of the fibronectin-fixed PHBV films were examined and compared with the original films, including -NH2 group expression, contact angle, mechanical properties, and fibronectin binding amount. Then, cytotoxicity and biocompatibility were measured by culture with L929 cells and endothelial progenitor cells (EPCs) of the fibronectin-fixed PHBV films. In addition, cell morphology, cell growth kinetics, acetylated low-density lipoprotein uptake ability, lectin binding ability and specific gene expressions of cultured EPCs on fibronectin-fixed PHBV films were also analyzed. Taken together, our data demonstrated that the surface of the aligned PHBV films could be successfully modified to immobilize fibronectin. Importantly, EPCs cultured on the fibronectin-fixed PHBV films showed excellent cell biocompatibility, a rapid proliferation rate, an aligned growth direction and correct cell functions. We believed that fibronectin-fixed PHBV films can serve as a potential scaffold for vascular tissue engineering.

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Acknowledgements

This work was supported by the Ministry of Science and Technology, Taiwan, Republic of China (MOST 108-2628-E-006-009-MY3) and Taoyuan General Hospital, Ministry of Health and Welfare, Taoyuan, Taiwan, Republic of China (PTH111033).

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Authors and Affiliations

  1. Department of Chemical Engineering and Materials Science, Yuan Ze University, Chung-Li, Taoyuan City, 32003, Taiwan

    Chien-Ning Hsu, Ya-Ting Lin, Shinn-Gwo Hong & Chao-Ling Yao

  2. Department of Orthopedics Surgery, Taoyuan General Hospital, Ministry of Health and Welfare, Taoyuan Dist., Taoyuan City, 33004, Taiwan

    Chien-Ning Hsu & Yu-Hsu Chen

  3. Department of Biology and Anatomy, National Defense Medical Center, Neihu Dist., Taipei City, 11490, Taiwan

    Yu-Hsu Chen

  4. Department of Chemical Engineering, National Cheng Kung University, East Dist., Tainan City, 70101, Taiwan

    Tsung-Yu Tseng & Chao-Ling Yao

  5. Department of Obstetrics and Gynecology, National Cheng Kung University Hospital, East Dist., Tainan City, 70146, Taiwan

    Hsing-Fen Tsai

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  1. Chien-Ning Hsu
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Hsu, CN., Lin, YT., Chen, YH. et al. An Aligned Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Scaffold Fixed with Fibronectin to Enhance the Attachment and Growth of Human Endothelial Progenitor Cells. Biotechnol Bioproc E 28, 428–438 (2023). https://doi.org/10.1007/s12257-022-0255-x

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