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Metabolic Engineering and Synthetic Biology of Cyanobacteria for Carbon Capture and Utilization

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Abstract

Cyanobacteria are photosynthetic bacteria that can directly convert CO2 into several bio-products for industrial use, such as alcohols and diols, terpenes, acids, and sugars. However, some of these compounds are either produced in low amounts or are absent in cyanobacteria, and thus strain development is required to achieve optimal production of these bio-products for industrial applications. Metabolic engineering has been applied to modify cyanobacteria for enhanced production of value-added compounds. In this review, we have elucidated metabolic engineering of various pathways with the yield and productivity of the desired products. We have also described recent overall strategies in advanced metabolic engineering.

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Acknowledgments

The authors would like to thank Sun Young Choi, Mieun Lee and Jigyeong Son for their valuable suggestions.

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Authors and Affiliations

  1. Department of Food Science and Biotechnology, Sungkyunkwan University (SKKU), Suwon, 16419, Korea

    Napisa Pattharaprachayakul & Han Min Woo

  2. Program in Biotechnology, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, Pathumwan, Bangkok, 10330, Thailand

    Napisa Pattharaprachayakul

  3. Department of Biotechnology and Bioengineering, Chonnam National University, Gwangju, 61186, Korea

    Napisa Pattharaprachayakul & Jong-il Choi

  4. Laboratory of Cyanobacterial Biotechnology, Department of Biochemistry, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, Pathumwan, Bangkok, 10330, Thailand

    Aran Incharoensakdi

  5. BioFoundry Research Center, Institute of Biotechnology and Bioengineering, Sungkyunkwan University (SKKU), Suwon, 16419, Korea

    Han Min Woo

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  1. Napisa Pattharaprachayakul
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  2. Jong-il Choi
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Correspondence to Han Min Woo.

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Author Contribution Statement|N.P. and H.M.W designed the outline of the article. N.P., J.-I.C, and HMW analyzed the references. N.P., J.-I.C, A.I. and HMW wrote the manuscript. All authors read and approved the manuscript.

Funding|This work was supported by Korea CCS R&D Center (KCRC) (2017M1A8A1072034) and Basic Science Research Program (2020R1F1A1048292) by the National Research Foundation of Korea, funded by the Korean Government (Ministry of Science and ICT). In addition, J.-I.C. received support from the Golden Seed Project (213008-05-5-SB910) grant and was funded by the Ministry of Agriculture and the Ministry of Oceans and Fisheries. N.P is the recipient of a student fellowship from the Royal Golden Jubilee Ph.D. Program.

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Pattharaprachayakul, N., Choi, Ji., Incharoensakdi, A. et al. Metabolic Engineering and Synthetic Biology of Cyanobacteria for Carbon Capture and Utilization. Biotechnol Bioproc E 25, 829–847 (2020). https://doi.org/10.1007/s12257-019-0447-1

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  • DOI: https://doi.org/10.1007/s12257-019-0447-1

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