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Effect of media components and morphology of Bacillus natto on menaquinone-7 synthesis in submerged fermentation

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Abstract

Vitamin K2 (menaquinone or MK) plays an important role in blood clotting, cardiovascular disease, and anti-osteoporosis. A novel bacterial strain was isolated and identified as Bacillus natto based on 16SrDNA sequencing and LC-MS analysis. The objective of this study was to improve the extraction efficiency and productivity of MK-7 from B. natto. Acid-heating method efficiently disrupted B. natto cells for MK-7 extraction. Bacillus natto had a wide range of pH (5.0 ~ 9.0) for optimal growth. Its MK-7 yield was increased when rotation speed was increased to 200 rpm. The highest MK-7 yield was obtained when glycerol and soy peptone were used in the growth media. Batch fermentation was subsequently tested in 5 L bioreactor, which gave a high productivity of MK-7 (at 0.60 mg/L/h). A positive correlation between MK-7 yield and sporulation ratio was also found. This study provides valuable information on the extraction and production of menaquinone-7 from B. natto under submerged fermentation condition.

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Authors and Affiliations

  1. Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), College of Biotechnology and Pharmaceutical Engineering, School of Pharmacy, Nanjing Tech University, Nanjing, 211-816, China

    Miao-miao Luo, Lu-jing Ren, Sheng-lan Chen, Xiao-jun Ji & He Huang

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  1. Miao-miao Luo
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  2. Lu-jing Ren
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  3. Sheng-lan Chen
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  4. Xiao-jun Ji
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  5. He Huang
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Correspondence to Lu-jing Ren or He Huang.

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Luo, Mm., Ren, Lj., Chen, Sl. et al. Effect of media components and morphology of Bacillus natto on menaquinone-7 synthesis in submerged fermentation. Biotechnol Bioproc E 21, 777–786 (2016). https://doi.org/10.1007/s12257-016-0202-9

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  • DOI: https://doi.org/10.1007/s12257-016-0202-9

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