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Designing of an Intensification Process for Biosynthesis and Recovery of Menaquinone-7

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Abstract

A nutritional food rich in menaquinone-7 has a potential in preventing osteoporosis and cardiovascular diseases. The static fermentation of Bacillus subtilis natto is widely regarded as an optimum process for menaquinone-7 production. The major issues for the bulk production of menaquinone-7 are the low fermentation yield, biofilm formation and the use of organic solvents for the vitamin extraction. In this study, we demonstrate that the dynamic fermentation involving high stirring and aeration rates enhances the yield of fermentation process significantly compared to static system. The menaquinone-7 concentration of 226 mg/L was produced at 1,000 rpm, 5 vvm, 40 °C after 5 days of fermentation. This concentration is 70-fold higher than commercially available food products such as natto. Additionally, it was found that more than 80 % of menaquinone-7 was recovered in situ in the vegetable oil that was gradually added to the system as an anti-foaming agent. The intensification process developed in this study has a capacity to produce an oil rich in menaquinone-7 in one step and eliminate the use of organic solvents for recovery of this compound. This oil can, therefore, be used for the preparation of broad range of supplementary and dietary food products rich in menaquinone-7 to reduce the risk of osteoporotic fractures and cardiovascular diseases.

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Acknowledgments

The authors acknowledge the financial support by the Australian Research Council (LP100100347) and Agricure Scientific Organics. RM also acknowledges the financial support of Henry Bertie and Florence Mabel Gritton scholarship.

Conflict of Interest

The authors report no conflicts of interest.

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Authors and Affiliations

  1. School of Chemical and Biomolecular Engineering, The University of Sydney, Sydney, NSW 2006, Australia

    Aydin Berenjian, Raja Mahanama, John Kavanagh & Fariba Dehghani

  2. Agricure Scientific Organics, Lot 6 Gantry, PI, Braemar, NSW 2575, Australia

    Andrea Talbot & Hubert Regtop

Authors
  1. Aydin Berenjian
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  2. Raja Mahanama
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  3. Andrea Talbot
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  4. Hubert Regtop
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  5. John Kavanagh
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  6. Fariba Dehghani
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Correspondence to Aydin Berenjian.

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Berenjian, A., Mahanama, R., Talbot, A. et al. Designing of an Intensification Process for Biosynthesis and Recovery of Menaquinone-7. Appl Biochem Biotechnol 172, 1347–1357 (2014). https://doi.org/10.1007/s12010-013-0602-7

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  • DOI: https://doi.org/10.1007/s12010-013-0602-7

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