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Introgression of Genes for Resistance against Phytophthora infestans in Diploid Potato

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Abstract

Potato is playing an increasingly important role in food production. The development of new varieties is slow due to the genetic complexity of potato and the inefficient breeding process. Modern techniques, such as hybrid breeding and the introduction of new traits in specific, existing elite material, have not been reported in the development of new and improved potato varieties. This paper describes the first example of marker-assisted introgression of four different Phytophthora infestans resistance genes in selected highly homozygous, diploid potato lines. After two backcrosses and one selfing, the original line can be recovered with Phytophthora resistance, thus providing added value. After crossing two diploid lines, each with a different resistance gene, hybrids were obtained and tested for resistance to Phytophthora in small field trials. In these experiments, the hybrids with two resistance genes were more resistant than the plants with only one of the two resistance genes.

Resumen

La papa esta jugando un papel en aumento importante en la producción de alimentos. El desarrollo de nuevas variedades es lento debido a la complejidad genética de la papa y al proceso ineficiente de mejoramiento. Las técnicas modernas, como el mejoramiento de híbridos y la introducción de nuevos caracteres en material élite específico existente, no se ha reportado en el desarrollo de nuevas variedades mejoradas. Este artículo describe el primer ejemplo de la introgresión asistida con marcadores de cuatro genes diferentes de resistencia a Phytophthora infestans en líneas seleccionadas altamente homozigóticas de papa diploide. Después de dos retrocruzas y una endogámica, se puede recuperar la línea original con resistencia a Phytophthora, y por ende, proporcionando un valor agregado. Después de cruzar dos líneas diploides, cada una con un gen diferente de resistencia, se obtuvieron híbridos y se probaron para resistencia a Phytophthora en pequeños ensayos de campo. En estos experimentos, los híbridos con dos genes de resistencia fueron más resistentes que las plantas con solo uno de los dos.

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Acknowledgements

This research was made possible by a Horizon 2020 grant (agreement 666729). The Wageningen University & Research Plant Breeding department provided pollen of the four wild relatives. HLB (Research and Consultancy in Agriculture; www.hlbbv.nl) supervised one of the Phytophthora trails, and the van Haeringen laboratory supported accurate and strictly timed KASP determinations. The experiments in this paper comply with the current laws of the Netherlands.

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Authors and Affiliations

  1. Solynta, Dreijenlaan 2, 6703HA, Wageningen, the Netherlands

    Ying Su, Marcela Viquez-Zamora, Danielle den Uil, Jarno Sinnige, Hein Kruyt, Pim Lindhout & Sjaak van Heusden

  2. Wageningen University & Research, Plant Breeding, 6708PB, Wageningen, the Netherlands

    Jack Vossen

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  1. Ying Su
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  2. Marcela Viquez-Zamora
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  3. Danielle den Uil
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Correspondence to Sjaak van Heusden.

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Su, Y., Viquez-Zamora, M., den Uil, D. et al. Introgression of Genes for Resistance against Phytophthora infestans in Diploid Potato. Am. J. Potato Res. 97, 33–42 (2020). https://doi.org/10.1007/s12230-019-09741-8

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  • DOI: https://doi.org/10.1007/s12230-019-09741-8

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