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Newly derived GH43 gene from compost metagenome showing dual xylanase and cellulase activities

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Abstract

A metagenomic fosmid library was constructed from compost microbial communities that were collected from various farms throughout the Khon Kaen province, Thailand. The library was enriched in carboxymethylcellulose (CM-cellulose)—containing media prior to the screening of clones capable of degrading cellulosic materials. Two clones were selected for further subcloning and sequencing based on different patterns from restriction analysis. Deduced amino acid analysis of possible ORFs revealed one novel gene encoding an enzyme belonging to glycosyl hydrolase family 43 (GH43), which is a GH family rarely found in metagenomic studies. The most notable finding is that this enzyme, designated as Biof1_09, shows dual activities, namely endocellulase and endoxylanase activities. Biof1_09 showed greater than 50 % of its activity under acidic conditions ranging from pH 3.5 to 5.5 with a pH optimum of 4.5. The optimum temperature of this enzyme was between 45 and 55 °C with an optimum of 50 °C. The properties of Biof1_09 make this enzyme an attractive candidate for large-scale expression for use in lignocellulose degradation for various bioprocess applications, including bioethanol fermentation.

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Acknowledgments

This research was supported by the Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission, through the Biofuel Cluster of Khon Kaen University. The authors thank the KKU Publication Clinic for providing professional editing service from American Journal Experts. We would also like to thank Dr. Piyanun Harnpicharnchai for the critical discussions and Miss Rinrapee Ngamsaeng and Miss Charinthip Suksart for the technical assistance. We are also grateful to the Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Khon Kaen University, Khon Kaen, Thailand, for the additional laboratory supports.

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  1. Department of Microbiology, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand

    Ritthironk Sae-Lee & Atcha Boonmee

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  1. Ritthironk Sae-Lee
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Correspondence to Atcha Boonmee.

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Sae-Lee, R., Boonmee, A. Newly derived GH43 gene from compost metagenome showing dual xylanase and cellulase activities. Folia Microbiol 59, 409–417 (2014). https://doi.org/10.1007/s12223-014-0313-7

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