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In vitro propagation and synseed production of scarlet salvia (Salvia splendens)

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Abstract

The present study provides a protocol for high-frequency in vitro propagation and short-term conservation using encapsulation technology of Salvia splendens. Nodal segments were more responsive than shoot tips in terms of shoot multiplication. Murashige and Skoog’s (MS) basal medium augmented with 5.0 µM 6-benzyl adenine (BA) was optimum for shoot induction through both explants. MS medium supplemented with 5.0 µM BA and 0.5 µM α-naphthalene acetic acid induced a maximum of 15.0 shoots per nodal segment and 5.0 cm shoot length after 4 weeks of incubation. Microshoots were best rooted (30.6 roots per shoot) on half-strength MS basal medium containing 2.5 µM indole-3-acetic acid (IAA). For synseed preparation, nodal segments were encapsulated in 4 % Na-alginate with different gel matrices and 100 mM CaCl2·2H2O (complexing agent). Maximum conversion (63.6 %) of synseeds into plantlets was achieved on MS basal medium when they were prepared with the gel matrix of MS basal medium supplemented with 5.0 µM BA and 2.5 µM IAA. Pretreatment of synseeds with 100 mM KNO3 for 30 min, prior to incubation on the planting media significantly improved conversion frequency (94.6 %). Synseeds could be stored up to 10 weeks at low temperature (4 °C). Complete plantlets were successfully acclimatized and established in field.

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Abbreviations

BA:

6-Benzyladenine

DDW:

Double distilled water

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

2-iP:

2-Isopentyl adenine

Kn:

Kinetin

KNO3 :

Potassium nitrate

MS:

Murashige and Skoog (1962)

NAA:

α-Naphthalene acetic acid

PGR:

Plant growth regulator

TDZ:

Thidiazuron

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Acknowledgments

Dr. Shiwali Sharma is thankful to DST, for the award of Young Scientist under Fast Track Scheme, SERB (vide no. SB/FT/LS-364/2012) for providing research assistance. Dr. Anwar Shahzad gratefully acknowledges the financial support provided by UGC and UP-CST in the form of research projects (vide No. 39-369/2010 SR and vide No. CST/D3836).

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Authors and Affiliations

  1. Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, Aligarh, 202 002, UP, India

    Shiwali Sharma, Anwar Shahzad, Jyotsana Kumar & Mohammad Anis

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  1. Shiwali Sharma
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  2. Anwar Shahzad
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  3. Jyotsana Kumar
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  4. Mohammad Anis
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Correspondence to Shiwali Sharma or Anwar Shahzad.

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Sharma, S., Shahzad, A., Kumar, J. et al. In vitro propagation and synseed production of scarlet salvia (Salvia splendens). Rend. Fis. Acc. Lincei 25, 359–368 (2014). https://doi.org/10.1007/s12210-014-0308-y

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