Abstract
A sensitive method has been established by using activated carbon as an adsorbent for extraction of indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) from bean sprout, tomato, potato, cucumber, and wheat grain prior to field-amplified sample injection-micellar electrokinetic capillary chromatography (FASI-MEKC) detection. Parameters affecting FASI and solid-phase extraction (SPE) were optimized in detail. Under the optimal conditions, the sensitivity of SPE-FASI-MEKC for IAA and IBA was respectively improved by 32.5 and 29.7 times as compared with the direct MEKC. The detection limits were 4.1 for IAA and 5.3 ng mL−1 for IBA with 4.9–5.2% RSD (n = 9, C = 0.03 μg mL−1). The new method has been successfully applied to detect IAA and IBA in bean sprout, tomato, potato, cucumber, and wheat grain with the recoveries of 81.7–110%.
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Funding
This project was financially supported by the Education Committee of Hubei Province (D20132501) and Hubei Key Laboratory of Pollutant Analysis and Reuse Technique (Hubei Normal University) (PA150208).
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Yang Zheng declares that he has no conflict of interest. Xiaofeng Peng declares that he has no conflict of interest. Yiwei Wu declares that he has no conflict of interest.
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Zheng, Y., Peng, X. & Wu, Y. Field-Amplified Sample Injection and Solid-Phase Extraction for Sensitivity Improvement of Electrophoretic Determination of Indole-3-Acetic Acid and Indole-3-Butyric Acid in Food Samples. Food Anal. Methods 11, 1155–1162 (2018). https://doi.org/10.1007/s12161-017-1091-z
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DOI: https://doi.org/10.1007/s12161-017-1091-z