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Determination of Ochratoxin A in Pig Muscle Using Dispersive Liquid-liquid Microextraction Combined with High-Performance Liquid Chromatography

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Abstract

A new method was developed for the rapid analysis of ochratoxin A (OTA) in pig muscle using dispersive liquid-liquid microextraction (DLLME) combined with high-performance liquid chromatography (HPLC). OTA was extracted with methanol and then cleaned up with DLLME procedure. After being blown dry and redissolved, the sample was determined using high-performance liquid chromatography with fluorescence detector (HPLC-FLD). Under the experimental conditions, the LOD was 0.21 μg kg−1, LOQ was 0.70 μg kg−1, and the recovery was higher than 80 %. Intra- and inter-day repeatability expressed as RSD were less than 5 %. The established method is more efficient, easier, and cheaper than conventional cleaning up procedure.

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Authors and Affiliations

  1. School of Public Health, Shandong University, Jinan, Shandong, China

    Chuanlei Luan, Lin Wang, Fangfang Chen, Shue Wang, Li Zhao & Lihua Shao

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  1. Chuanlei Luan
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  2. Lin Wang
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  3. Fangfang Chen
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  4. Shue Wang
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  5. Li Zhao
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  6. Lihua Shao
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Correspondence to Lihua Shao.

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Chuanlei Luan declares that he has no conflict of interest, Lin Wang declares that he has no conflict of interest, Fangfang Chen declares that she has no conflict of interest, Shue Wang declares that she has no conflict of interest, Li Zhao declares that she has no conflict of interest, Lihua Shao declares that she has no conflict of interest.

This article does not contain any studies with human participants performed by any of the authors.

Informed consent was obtained from all individual participants included in the study.

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Luan, C., Wang, L., Chen, F. et al. Determination of Ochratoxin A in Pig Muscle Using Dispersive Liquid-liquid Microextraction Combined with High-Performance Liquid Chromatography. Food Anal. Methods 9, 1490–1494 (2016). https://doi.org/10.1007/s12161-015-0330-4

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  • DOI: https://doi.org/10.1007/s12161-015-0330-4

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