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Determination of ochratoxin A in Portuguese rice samples by high performance liquid chromatography with fluorescence detection

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Abstract

A sensitive and accurate analytical method for the determination of ochratoxin A (OTA) in rice, based on extraction with phosphate-buffered saline/methanol, an immunoaffinity column (IAC) for clean-up, and high performance liquid chromatography with fluorescence detection (HPLC-FD), is described. The limit of quantification of the proposed method was 0.05 μg kg−1. Recovery of OTA from rice samples spiked at 0.05 μg kg−1 was 92%, with a within-day RSD of 5.4%. The proposed method was applied to 42 rice samples from Portugal and the presence of OTA was found in six samples at concentrations ranging from 0.09 to 3.52 μg kg−1. The identification of OTA was confirmed by methyl ester derivatization and then HPLC analysis. The daily intake of OTA by the Portuguese population was also estimated.

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Acknowledgements

We gratefully thank FCT and POCTI (FEDER) and Integrated Action no. E-1/05 for financial support of this study.

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Authors and Affiliations

  1. Group of Bromatology, Faculty of Pharmacy, University of Coimbra, 3049 Coimbra, Portugal

    A. Pena, F. Cerejo, C. Lino & I. Silveira

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  1. A. Pena
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  2. F. Cerejo
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  3. C. Lino
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  4. I. Silveira
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Correspondence to A. Pena.

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Pena, A., Cerejo, F., Lino, C. et al. Determination of ochratoxin A in Portuguese rice samples by high performance liquid chromatography with fluorescence detection. Anal Bioanal Chem 382, 1288–1293 (2005). https://doi.org/10.1007/s00216-005-3254-9

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  • DOI: https://doi.org/10.1007/s00216-005-3254-9

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