Abstract
Musashi1 (Msi1) is an RNA-binding protein that is involved in cell fate determination. Here, we report the 1H, 15N, and 13C resonance assignments of Msi1 second RNA-binding domain in free form and in complex with RNA. The assignments can be utilized for NMR structure and dynamics analyses of the Msi1:RNA complex, and moreover, for chemical shift perturbation analyses to evaluate the binding of potential small molecule inhibitors against Msi1:RNA interaction.
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Acknowledgements
This work was supported by Japan Society for the Promotion of Science KAKENHI to M.K. (#15H01256, #16H00833, and #16K14678) and to T.N. (#15H01634 and #26440026). The authors would like to acknowledge Dr. Mikako Shirouzu for fruitful discussion.
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Iwaoka, R., Nagata, T., Tsuda, K. et al. Backbone and side chain assignments of the second RNA-binding domain of Musashi-1 in its free form and in complex with 5-mer RNA. Biomol NMR Assign 11, 265–268 (2017). https://doi.org/10.1007/s12104-017-9760-9
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DOI: https://doi.org/10.1007/s12104-017-9760-9