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Clinicoepidemiological Profile and Genetic Characterization of Circulating Rotavirus Strain among Children < 5 Years Hospitalized for Acute Gastroenteritis in Western Rajasthan, India

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Abstract

Objective

To determine the clinical and epidemiological profile and circulating strains of rotavirus among children less than 5 y of age hospitalized for diarrhea in a tertiary care center of western Rajasthan.

Method

Children < 5 y of age admitted for acute gastroenteritis were recruited in this hospital-based surveillance study. Detailed clinical history and sociodemographic information was collected for all enrolled children. Severity of the gastroenteritis was assessed using the 20-point Vesikari Clinical Severity Scoring System. Rotavirus positivity was tested in the stool samples collected from children by commercially available techniques and further molecular characterization done as per defined protocol.

Results

Out of 1055 enrolled children, overall positivity rate of typable rotavirus was 18.95% (169/892). Among children who were less than 24 mo of age, positivity due to rotavirus diarrhea was maximum (24.4%). Distinct seasonality was observed as maximum cases of rotavirus diarrhea were detected in the months of December to February. Commonest G type was G3 (54%) followed by G1 (19%) while predominant P type was P[8] (77%) followed by P[4] (11%). G3P[8] (51.83%) was the commonest genotype observed in the study region.

Conclusion

The current study found positivity of rotavirus to be 18.95% among children aged 0–59 mo admitted due to acute gastroenteritis is a tertiary care setting in northern part of India. Emergence of newer predominant strains emphasizes the need of continued surveillance to determine the changing trends.

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Acknowledgements

The study was supported by the Indian Council of Medical Research, New Delhi; Ministry of Health and Family Welfare, Govt. of India and the State government of the participating institution.

The authors thank the team at Christian Medical College, Vellore for support, and the children and families for participation. The authors wish to acknowledge the contribution of Dr. Raksha Sharma (Medical Officer) for her support.

Funding

This work was supported by grants from the Bill and Melinda Gates Foundation to the Centers for Disease Control and Prevention, Atlanta, GA, USA (subcontract to Christian Medical College, Vellore grant no MOA#871-15SC) and the Translational Health Science and Technology Institute (grant no OPP1165083).

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Authors and Affiliations

  1. Department of Pediatrics, Dr S N Medical College, Jodhpur, Rajasthan, 342003, India

    Pramod Sharma, Suresh Kumar Meel, Vikash Katewa, Alka Bishnoi & Vijay Kumar Verma

  2. Department of Obstetrics & Gynaecology, Dr S N Medical College, Jodhpur, Rajasthan, India

    Suman Katewa

  3. The Wellcome Trust Research Laboratory, Christian Medical College, Vellore, Tamil Nadu, India

    Nayana P. Nair & Varunkumar Thiyagarajan

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  1. Pramod Sharma
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Contributions

PS contributed to conception, design, and critically revision of the manuscript; SKM, AB, VKV contributed to recruitment of the participants and data acquisition, VK contributed to design, data interpretation, analysis, and drafting of article; NPN and VT analyzed the data; SK contributed in manuscript writing, data analysis and critically revision of the manuscript. All authors critically revised and approved the final manuscript. PS will act as guarantor for this paper.

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Correspondence to Vikash Katewa.

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Sharma, P., Katewa, S., Meel, S.K. et al. Clinicoepidemiological Profile and Genetic Characterization of Circulating Rotavirus Strain among Children < 5 Years Hospitalized for Acute Gastroenteritis in Western Rajasthan, India. Indian J Pediatr 88 (Suppl 1), 97–104 (2021). https://doi.org/10.1007/s12098-020-03628-x

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