Abstract
Background and aim
Long non-coding RNAs (lncRNAs) have been demonstrated to act as a critical regulator in the processes of tumor biology. In this study, whether lncRNA-ATB is a potential indicator for non-small cell lung cancer (NSCLC) was investigated and its biological function in NSCLC was also determined.
Methods
The expression levels of lncRNA-ATB in NSCLC tissues and cell lines were measured. A549 cell line was explored to investigate the functions of lncRNA-ATB in NSCLC.
Results
Real-time PCR results showed that lncRNA-ATB expression was up-regulated in both in NSCLC tissues and cell lines. High lncRNA-ATB expression in tumor tissue was associated with larger tumor size, lymph node metastasis, and distant metastasis in patients with NSCLC, respectively. In addition, the patients with high expression of lncRNA-ATB presented a lower survival probability. In vitro experiments showed that down-regulation of lncRNA-ATB promoted the cell apoptosis, whereas inhibited the cell viability, cell migration, and cell invasion.
Conclusion
High expression of lncRNA-ATB indicated a poor prognosis and led to the cell proliferation and metastasis in NSCLC.
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Written informed consents were obtained from all the participants, and this study was approved by the Ethics Committee of Anhui Provincial Hospital.
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12094_2016_1572_MOESM1_ESM.jpg
Effects of lncRNA-ATB down-regulation on cell viability of NCI-H1299 cells. a Transfection efficiency was confirmed by determining the expression of lncRNA-ATB after transfection of si-ATB (Mock acting as control). b Cell viability was evaluated after transfection. ** P<0.01, vs. Mock (JPEG 551 kb)
12094_2016_1572_MOESM2_ESM.jpg
Effects of lncRNA-ATB down-regulation on cell migration and invasion of NCI-H1299 cells. NCI-H1299 cells were transfected with si-ATB (Mock acting as control). a Cell migration and b cell invasion were evaluated after transfection. ** P<0.01, vs. Mock 2 (JPEG 330 kb)
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Ke, L., Xu, SB., Wang, J. et al. High expression of long non-coding RNA ATB indicates a poor prognosis and regulates cell proliferation and metastasis in non-small cell lung cancer. Clin Transl Oncol 19, 599–605 (2017). https://doi.org/10.1007/s12094-016-1572-3
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DOI: https://doi.org/10.1007/s12094-016-1572-3