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Diagnosis of Campylobacter spp. Isolates and Their Antimicrobial Susceptibility Patterns

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Abstract

This study aimed to reveal antibiotic resistance patterns and molecular characterization of quinolone resistance Campylobacter isolates in patients with diarrhea. Campylobacter spp. isolated from 35.33% of the total samples, most of which were from male patients aged 3 months to 10 years. Identifying isolates at the species level made in MALDI-TOF MS, 82.4% were C. jejuni, and 17.6% were C. coli. Respectively 94% (47/50), 58% (29/50), and 2% (1/50) resistance rates were determined for ciprofloxacin, tetracycline, and erythromycin. While C. jejuni isolates were more resistant to ciprofloxacin and tetracycline than C. coli, they showed no resistance to erythromycin. Quinolone resistance determining region (QRDR) were evaluated by mismatch amplification mutation test and all quinolone resistant strains gave positive results. One of the seven silent mutations identified was specific to this study, and two other novel mutations were also identified.

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Acknowledgements

We would also like to thank Prof. Dr. Semih Yılmaz, Abeer Babiker, Naima Sirad, and Sümeyye Temizgül for their contributions. The studies were conducted in the Bacteriology Laboratory of the Faculty of Medicine and the laboratories of the Faculty of Science and Faculty of Agriculture, Erciyes University. We specially thank Erciyes University Editing office for language improvement.

Funding

This work was supported by the Erciyes University Research Projects Unit (Grand Number: FYL-2022-12124).

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Authors and Affiliations

  1. Graduate School of Natural and Applied Science, Erciyes University, Kayseri, Turkey

    Hussein Ali Kamil Alobaidy

  2. Department of Biology, Faculty of Science, Erciyes University, Kayseri, Turkey

    Abdurrahman Ayvaz

  3. Department of Medical Microbiology, School of Medicine, Erciyes University, Kayseri, Turkey

    Pınar Sağiroğlu & Mustafa Altay Atalay

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  1. Hussein Ali Kamil Alobaidy
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Contributions

HAKA conceived and performed the experiment and contributed to the analysis, AA was involved in processing the experimental data, performed the analysis, drafted the manuscript. PS and MAA aided in interpreting the results, all authors provided critical feedback and helped shape the research, analysis, and manuscript.

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Correspondence to Abdurrahman Ayvaz.

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Alobaidy, H.A.K., Ayvaz, A., Sağiroğlu, P. et al. Diagnosis of Campylobacter spp. Isolates and Their Antimicrobial Susceptibility Patterns. Indian J Microbiol (2024). https://doi.org/10.1007/s12088-024-01199-5

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