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Green Synthesis of Silver Nanoparticles Using Extracts of Cocculus Pendulus: Morphology and Antibacterial Efficacy Against Common Nosocomial Pathogens

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Abstract

The use of plant extract to synthesize nanoparticles has transformed the area of nanotechnology. Silver nanoparticles (AgNPs) were generated in this work using the leaves of Cocculus pendulus as a reducing and stabilizing agent. The extract of C. pendulus was analyzed using a gas chromatography-mass spectrometer. UV-visible spectroscopy (UV-visible), Fourier Transform Infrared, Zeta potential, and Transmission Electron Microscopy were used to analyze the bio-fabricated AgNPs. In addition, we describe the antibacterial and antioxidant characteristics of AgNPs produced. Analyses were used to describe the morphology of produced AgNPs, which had a typical size of 45 nm. AgNP was discovered to be extremely poisonous to gram-positive bacteria and was a promising antibiotic against bacterial infections. Furthermore, antioxidant assays revealed the greatest free radical scavenging activity. Finally, our findings show that C. pendulus can be used as a source for green synthesis of AgNPs with strong antioxidant and antibacterial properties in vitro.

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Data Availability

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

References

  1. Balciunaitiene A, Viskelis P, Viskelis J, Streimikyte P, Liaudanskas M, Bartkiene E et al (2021) Green synthesis of silver nanoparticles using extract of Artemisia absinthium L., Humulus lupulus L. and Thymus vulgaris L., physico-chemical characterization, antimicrobial and antioxidant activity. Processes 9:1304

    Article  CAS  Google Scholar 

  2. Salayová A, Bedlovičová Z, Daneu N, Baláž M, Lukáčová Bujňáková Z, Balážová Ľ et al (2021) Green synthesis of silver nanoparticles with antibacterial activity using various medicinal plant extracts: morphology and antibacterial efficacy. Nanomaterials 11:1005

    Article  PubMed  PubMed Central  Google Scholar 

  3. Krithiga N, Rajalakshmi A, Jayachitra A (2015) Green synthesis of silver nanoparticles using leaf extracts of Clitoria ternatea and Solanum nigrum and study of its antibacterial effect against common nosocomial pathogens. J Nanosci 2015:1–8

    Article  Google Scholar 

  4. Salem SS, Fouda A (2021) Green synthesis of metallic nanoparticles and their prospective biotechnological applications: an overview. Biol Trace Elem Res 199:344–370

    Article  CAS  PubMed  Google Scholar 

  5. Kambale EK, Nkanga CI, Mutonkole B-PI, Bapolisi AM, Tassa DO, Liesse J-MI et al (2020) Green synthesis of antimicrobial silver nanoparticles using aqueous leaf extracts from three Congolese plant species (Brillantaisia Patula, Crossopteryx febrifuga and Senna siamea). Heliyon 6:e04493

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Melkamu WW, Bitew LT (2021) Green synthesis of silver nanoparticles using Hagenia Abyssinica (Bruce) JF Gmel plant leaf extract and their antibacterial and anti-oxidant activities. Heliyon 7:e08459

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Ghoshal G, Singh M (2022) Characterization of silver nano-particles synthesized using fenugreek leave extract and its antibacterial activity. Mater Sci Energy Technol 5:22–29

    CAS  Google Scholar 

  8. Mariselvam R, Ranjitsingh A, Nanthini AUR, Kalirajan K, Padmalatha C, Selvakumar PM (2014) Green synthesis of silver nanoparticles from the extract of the inflorescence of Cocos nucifera (Family: Arecaceae) for enhanced antibacterial activity. Spectrochim Acta A Mol Biomol Spectrosc 129:537–541

    Article  ADS  CAS  PubMed  Google Scholar 

  9. Nafees M, Ullah S, Javaid A (2020) Pharmacognostic and phytochemical screening of Cocculus Pendulus Diels. Stem and root. J Anim Plant Sci 30

  10. Sultani SZ, Anwar MA, Alam MS (2022) New Bisbenzylisoquinoline alkaloid, isolation and structure elucidation from Cocculus Pendulus. Nat Prod Res 1–7

  11. Lee YJ, Song K, Cha S-H, Cho S, Kim YS, Park Y (2019) Sesquiterpenoids from Tussilago farfara flower bud extract for the eco-friendly synthesis of silver and gold nanoparticles possessing antibacterial and anticancer activities. Nanomaterials 9:819

    Article  ADS  CAS  PubMed  PubMed Central  Google Scholar 

  12. Ramala SK, Alagumanivasagam G (2019) GC-MS analysis of phytoconstituents presents in ethanolic extract of plant Cocculus Pendulus (JR & G. Forst) diels. Int Res J Pharm 10:233–236

    Article  CAS  Google Scholar 

  13. Sarwer Q, Amjad MS, Mehmood A, Binish Z, Mustafa G, Farooq A et al (2022) Green synthesis and characterization of silver nanoparticles using Myrsine Africana leaf extract for their antibacterial, antioxidant and phytotoxic activities. Molecules 27:7612

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Alsammarraie FK, Wang W, Zhou P, Mustapha A, Lin M (2018) Green synthesis of silver nanoparticles using turmeric extracts and investigation of their antibacterial activities. Colloids Surf B Biointerfaces 171:398–405

    Article  CAS  PubMed  Google Scholar 

  15. Al-Otibi FO, Yassin MT, Al-Askar AA, Maniah K (2023) Green biofabrication of silver nanoparticles of potential synergistic activity with antibacterial and antifungal agents against some nosocomial pathogens. Microorganisms 11:945

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Ameena S, Rajesh N, Anjum SM, Khadri H, Riazunnisa K, Mohammed A et al (2022) Antioxidant, antibacterial, and anti-diabetic activity of green synthesized copper nanoparticles of Cocculus Hirsutus (Menispermaceae). Appl Biochem Biotechnol 194:4424–4438

    Article  CAS  PubMed  Google Scholar 

  17. Salari S, Bahabadi SE, Samzadeh-Kermani A, Yosefzaei F (2019) In-vitro evaluation of antioxidant and antibacterial potential of greensynthesized silver nanoparticles using Prosopis farcta fruit extract. Iran J Pharm Res 18:430

    CAS  PubMed  PubMed Central  Google Scholar 

  18. Ramala SK, Alagumanivasagam G (2019) Formulation and characterisation of nanosuspension of plant extract Cocculus Pendulus. J Glob Pharma Technol 11:25–29

    CAS  Google Scholar 

  19. Jameel MS, Aziz AA, Dheyab MA (2020) Green synthesis: proposed mechanism and factors influencing the synthesis of platinum nanoparticles. Green Process Synth 9:386–398

    Article  Google Scholar 

  20. Sattari R, Khayati GR (2020) Prediction of the size of silver nanoparticles prepared via green synthesis: a gene expression programming approach. Sci Iran 27:3399–3411

    Google Scholar 

  21. Szczyglewska P, Feliczak-Guzik A, Nowak I (2023) Nanotechnology–general aspects: a chemical reduction approach to the synthesis of nanoparticles. Molecules 28:4932

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Riaz M, Ismail M, Ahmad B, Zahid N, Jabbour G, Khan MS et al (2020) Characterizations and analysis of the antioxidant, antimicrobial, and dye reduction ability of green synthesized silver nanoparticles. Green Process Synth 9:693–705

    Article  Google Scholar 

  23. Mouzaki M, Maroui I, Mir Y, Lemkhente Z, Attaoui H, El Ouardy K et al (2022) Green synthesis of silver nanoparticles and their antibacterial activities. Green Process Synth 11:1136–1147

    Article  CAS  Google Scholar 

  24. Slavin YN, Asnis J, Häfeli UO, Bach H (2017) Metal nanoparticles: understanding the mechanisms behind antibacterial activity. J Nanobiotechnol 15:65

    Article  Google Scholar 

  25. Torabfam M, Yüce M (2020) Microwave-assisted green synthesis of silver nanoparticles using dried extracts of Chlorella vulgaris and antibacterial activity studies. Green Process Synth 9:283–293

    Article  Google Scholar 

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Acknowledgements

This research was supported by the Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran (grant no: 01s26).

Funding

This work was supported by a grant no: 01s26 from Vice Chancellor for Research, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

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Authors and Affiliations

  1. Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

    Zahra Asadi & Ali Ghaemi

  2. Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

    Morteza Saki, Mansour Amin & Sousan Akrami

  3. Department of Chemical Engineering, Sahand University of Technology, Tabriz, Iran

    Raheleh Khosravi

  4. Students’ Scientific Research Center (SSRC), Tehran University of Medical Sciences, Tehran, Iran

    Sousan Akrami

  5. Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Sousan Akrami

Authors
  1. Zahra Asadi
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  2. Morteza Saki
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  3. Raheleh Khosravi
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  4. Mansour Amin
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  5. Ali Ghaemi
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  6. Sousan Akrami
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Contributions

M.A., A.G. conceptual framework and technical oversight on all experiments; R.K. conducted the experiments and analyzed the experimental data. S.A. wrote the manuscript; M.S. revised the manuscript. All authors read and approved the final manuscript.

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Correspondence to Sousan Akrami.

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All authors declare that they do not have any conflict of interest.

Ethical Approval

The experiment plan and all methods were performed in accordance with the guidelines and regulations of Ahvaz Jundishapur University of Medical Sciences and approved by the institutional ethical committees. The present research was supported by the Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran (No. IR.AJUMS.REC.1401.184).

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Asadi, Z., Saki, M., Khosravi, R. et al. Green Synthesis of Silver Nanoparticles Using Extracts of Cocculus Pendulus: Morphology and Antibacterial Efficacy Against Common Nosocomial Pathogens. Indian J Microbiol (2024). https://doi.org/10.1007/s12088-023-01187-1

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