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Role of green fluorescent proteins and their variants in development of FRET-based sensors

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Abstract

Since the last decade, a lot of advancement has been made to understand biological processes involving complex intracellular pathways. The major challenge faced was monitoring and trafficking of metabolites in real time. Although a range of quantitative and imaging techniques have been developed so far, the discovery of green fluorescent proteins (GFPs) has revolutionized the advancement in the field of metabolomics. GFPs and their variants have enabled researchers to ‘paint’ a wide range of biological molecules. Fluorescence resonance energy transfer (FRET)-based genetically encoded sensors is a promising technology to decipher the real-time monitoring of the cellular events inside living cells. GFPs and their variants, due to their intrinsic fluorescence properties, are extensively being used nowadays in cell-based assays. This review focuses on structure and function of GFP and its derivatives, mechanism emission and their use in the development of FRET-based sensors for metabolites.

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Acknowledgements

The first author (NS) is thankful to University Grants Commission for a Junior Research Fellowship. Financial assistance in the form of a start-up research grant (Grant No. YSS/2014/000393/LS) from SERB, Department of Science and Technology, Government of India for conducting this research work is gratefully acknowledged.

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  1. Department of Biosciences, Jamia Millia Islamia, New Delhi, 110 025, India

    Neha Soleja, Ovais Manzoor & Mohd. Mohsin

  2. Faculty of Dentistry, Jamia Millia Islamia, New Delhi, 110 025, India

    Imran Khan

  3. Department of Botany, Aligarh Muslim University, Aligarh, India

    Altaf Ahmad

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  1. Neha Soleja
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  2. Ovais Manzoor
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Communicated by AMITABHA CHATTOPADHYAY.

Corresponding editor: Amitabha Chattopadhyay

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Soleja, N., Manzoor, O., Khan, I. et al. Role of green fluorescent proteins and their variants in development of FRET-based sensors. J Biosci 43, 763–784 (2018). https://doi.org/10.1007/s12038-018-9783-0

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