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Recent excitements in protein NMR: Large proteins and biologically relevant dynamics

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Abstract

The advent of Transverse Relaxation Optimized SpectroscopY (TROSY) and perdeuteration allowed biomolecular NMR spectroscopists to overcome the size limitation barrier (~20 kDa) in de novo structure determination of proteins. The utility of these techniques was immediately demonstrated on large proteins and protein complexes (e.g. GroEL-GroES, ClpP protease, Hsp90-p53, 20S proteasome, etc.). Further, recent methodological developments such as Residual Dipolar Couplings and Paramagnetic Relaxation Enhancement allowed accurate measurement of long-range structural restraints. Additionally, Carr-Purcell-Meiboom-Gill (CPMG), rotating frame relaxation experiments (R1ρ) and saturation transfer experiments (CEST and DEST) created never-before accessibility to the μs–ms timescale dynamic parameters that led to the deeper understanding of biological processes. Meanwhile, the excitement in the field continued with a series of developments in the fast data acquisition methods allowing rapid structural studies on less stable proteins. This review aims to discuss important developments in the field of biomolecular NMR spectroscopy in the recent past, i.e., in the post TROSY era. These developments not only gave access to the structural studies of large protein assemblies, but also revolutionized tools in the arsenal of today’s biomolecular NMR and point to a bright future of biomolecular NMR spectroscopy.

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Acknowledgements

This work was supported by Council of Scientific and Industrial Research (CSIR) network projects GenCODE (BSC0123) and SplenDID (BSC0104). CSC acknowledges a PhD fellowship from the CSIR, India.

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  1. CSIR-Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad, 500007, India

    Sai Chaitanya Chiliveri & Mandar V Deshmukh

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  1. Sai Chaitanya Chiliveri
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Correspondence to Mandar V Deshmukh.

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Corresponding editor: Durgadas P Kasbekar

[Chiliveri SC and Deshmukh MV 2016 Recent excitements in protein NMR: Large proteins and biologically relevant dynamics. J. Biosci.]

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Chiliveri, S.C., Deshmukh, M.V. Recent excitements in protein NMR: Large proteins and biologically relevant dynamics. J Biosci 41, 787–803 (2016). https://doi.org/10.1007/s12038-016-9640-y

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