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Protective Effects of Selenium, N-Acetylcysteine and Vitamin E Against Acute Ethanol Intoxication in Rats

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Abstract

The aim of this study was to determine possible protective influences of selenium (Se), N-acetylcysteine (NAC), and vitamin E (Vit E) against acute ethanol (EtOH) intoxication. Thirty-six rats were divided into six groups: I (control), II (EtOH), III (EtOH + Se), IV (EtOH + Vit E), V (EtOH + NAC), and VI (EtOH + mix). Except group I, EtOH was given the other pretreated (groups III, IV, V, and VI) and untreated groups (group II). Compared with the EtOH group, serum aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, lactate dehydrogenase, creatine kinase, and creatine kinase-MB levels were significantly decreased in all pretreated groups, whereas slightly diminished amylase and lipase were observed. Compared with the control group, a remarkably lower total antioxidant status (TAS), but higher total oxidant status (TOS), and oxidative stress index (OSI) were seen in brain, liver, and kidney tissues. The values of these parameters were less affected from EtOH-exposed brain tissue of EtOH + NAC and liver of EtOH + mix groups. Both significant decrease of catalase activity and marked increases of adenosine deaminase and myeloperoxidase were determined only in liver tissue of the EtOH group. Activities of these enzymes were restored in almost all pretreated groups. Moreover, an increase of xanthine oxidase activity was prevented in brain tissue of pretreated groups. In histopathological examination of the liver, hydropic degeneration, sinusoidal dilatation, mononuclear cell infiltration, and marked congestion, which were seen in the EtOH group, were prevented in all pretreated groups. Relative protection against acute EtOH toxicity, in both single and combined pretreatments of Se, NAC, and Vit E supplementation, was probably through antioxidant and free radical-neutralizing effects of foregoing materials.

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Authors and Affiliations

  1. Department of Medical Biology, Faculty of Medicine, Yuzuncu Yil University, Van, Turkey

    Halil Ozkol & Yasin Tuluce

  2. Department of Pathology, Faculty of Medicine, Yuzuncu Yil University, Van, Turkey

    Gulay Bulut

  3. Department of Biochemistry, Faculty of Medicine, Yuzuncu Yil University, Van, Turkey

    Ragıp Balahoroglu

  4. Department of Dermatology, Faculty of Medicine, Yuzuncu Yil University, Van, Turkey

    Hatice Uce Ozkol

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  1. Halil Ozkol
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  5. Hatice Uce Ozkol
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Correspondence to Hatice Uce Ozkol.

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All animals received humane care in compliance with the European community guidelines on the care and use of laboratory animals. This study was approved by the ethic committee of the Yuzuncu Yıl University (Decision number: 2015/12).

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The authors declare that they have no conflict of interest.

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Ozkol, H., Bulut, G., Balahoroglu, R. et al. Protective Effects of Selenium, N-Acetylcysteine and Vitamin E Against Acute Ethanol Intoxication in Rats. Biol Trace Elem Res 175, 177–185 (2017). https://doi.org/10.1007/s12011-016-0762-8

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  • DOI: https://doi.org/10.1007/s12011-016-0762-8

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