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Green Process for Producing Xylooligosaccharides by Using Sequential Auto-hydrolysis and Xylanase Hydrolysis

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Abstract

Xylooligosaccharides (XOS), as prebiotic oligomers, are increasingly receiving attention as high value-added products produced from lignocellulosic biomass. Although the XOS contains a series of different degrees of polymerization (DP) of xylose units, DP 2 and 3 (xylobiose (X2) and xylotriose (X3)) are regarded as the main active components in food and pharmaceutical fields. Therefore, in the study, in order to achieve the maximum production of XOS with the desired DP, a combination strategy of sequential auto-hydrolysis and xylanase hydrolysis was developed with corncob as raw material. The evidences showed that the hemicellulosic xylan could be effectively decomposed into various higher DP saccharides (> 4), which were dissolved into the auto-hydrolysate; sequentially, the soluble saccharides could be rapidly hydrolyzed into XOS with desired DP by xylanase hydrolysis. Finally, a maximum XOS yield of 56.3% was achieved and the ratio of (X2 + X3)/XOS was over 80%; meanwhile, the by-products could be controlled at lower levels. Overall, this study provides solid data that support the selective and precise preparation of XOS from corncob, vigorously promoting the application of XOS as functional sugar products.

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Acknowledgements

The authors acknowledge the support of the Advanced Analysis and Testing Center of Nanjing Forestry University.

Funding

This work was supported by the financial support from the Supported by the Opening Project of Guangxi Key Laboratory of Clean Pulp & Papermaking and Pollution Control, Nanning 530004, PR China (2021KF25) and Qing Lan Project of Jiangsu Province, China (SJCX22_0324).

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Authors and Affiliations

  1. Guangxi Key Laboratory of Clean Pulp & Papermaking and Pollution Control, School of Light Industry and Food Engineering, Guangxi University, Nanning, 530004, China

    Yujie Zhai & Shuangquan Yao

  2. Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, People’s Republic of China

    Yujie Zhai, Lei Zhang & Xin Zhou

  3. School of Basic Medical Sciences and Forensic Medicine, Hangzhou Medical College, Hangzhou, China

    Kankan Jiang

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  1. Yujie Zhai
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  4. Xin Zhou
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YZ and LZ performed the experiments, analyzed the data. XZ prepared the draft manuscript. SY and KJ reviewed and revised the manuscript. All authors read and approved the final manuscript.

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Correspondence to Xin Zhou or Kankan Jiang.

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Zhai, Y., Zhang, L., Yao, S. et al. Green Process for Producing Xylooligosaccharides by Using Sequential Auto-hydrolysis and Xylanase Hydrolysis. Appl Biochem Biotechnol (2023). https://doi.org/10.1007/s12010-023-04800-7

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