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Breeding of Saccharomyces cerevisiae with a High-Throughput Screening Strategy for Improvement of S-Adenosyl-L-Methionine Production

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Abstract

S-adenosyl-l-methionine (SAM), a vital physiologically active substance in living organisms, is produced by fermentation over Saccharomyces cerevisiae. The main limitation in SAM production was the low biosynthesis ability of SAM in S. cerevisiae. The aim of this work is to breed an SAM-overproducing mutant through UV mutagenesis coupled with high-throughput selection. Firstly, a high-throughput screening method by rapid identification of positive colonies was conducted. White colonies on YND medium were selected as positive strains. Then, nystatin/sinefungin was chosen as a resistant agent in directed mutagenesis. After several cycles of mutagenesis, a stable mutant 616–19-5 was successfully obtained and exhibited higher SAM production (0.41 g/L vs 1.39 g/L). Furthermore, the transcript levels of the genes SAM2, ADO1, and CHO2 involved in SAM biosynthesis increased, while ergosterol biosynthesis genes in mutant 616–19-5 significantly decreased. Finally, building on the above work, S. cerevisiae 616–19-5 could produce 10.92 ± 0.2 g/L SAM in a 5-L fermenter after 96 h of fermentation, showing a 2.02-fold increase in the product yield compared with the parent strain. Paving the way of breeding SAM-overproducing strain has improved the good basis for SAM industrial production.

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Data Availability

The datasets generated during and analyzed during the current study are available from the corresponding author on reasonable request.

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Funding

This research was supported by the National Key Research and Development Program of China (2022YFC2105400).

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Authors and Affiliations

  1. Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou, 310014, People’s Republic of China

    Zhong-Ce Hu, Yun-Chao Tao, Jun-Chao Pan, Chui-Mu Zheng, Yuan-Shan Wang, Ya-Ping Xue, Zhi-Qiang Liu & Yu-Guo Zheng

  2. The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou, 310014, People’s Republic of China

    Zhong-Ce Hu, Yun-Chao Tao, Jun-Chao Pan, Chui-Mu Zheng, Yuan-Shan Wang, Ya-Ping Xue, Zhi-Qiang Liu & Yu-Guo Zheng

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  1. Zhong-Ce Hu
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Contributions

Yun-Chao Tao: Conceptualization, Investigation, Data curation, Writing—original draft, Writing—review and editing. Zhong-Ce Hu: Funding acquisition, Conceptualization, Methodology, Supervision, Writing review and editing. Jun-Chao Pan, Chui-Mu Zheng: Conceptualization, Investigation, Data curation. Yuan-Shan Wang, Zhi-Qiang Liu, Ya-Ping Xue: Supervision. Yu-Guo Zheng: Validation, Visualization, Supervision, Conceptualization, Methodology.

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Correspondence to Zhi-Qiang Liu.

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Hu, ZC., Tao, YC., Pan, JC. et al. Breeding of Saccharomyces cerevisiae with a High-Throughput Screening Strategy for Improvement of S-Adenosyl-L-Methionine Production. Appl Biochem Biotechnol 196, 1450–1463 (2024). https://doi.org/10.1007/s12010-023-04622-7

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