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Novel Polyherbal Nanocolloids to Control Bovine Mastitis

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Abstract

Mastitis is a widespread disease in dairy cattle occurring throughout the world. The increased use of antibiotics brings about the development of antibiotic-resistant microbes. The application of antibiotics in dairy farming led to increased antibiotic resistance and represents a major obstacle for the treatment of mastitis. Recent advancements in nanotechnology led to the development of nanocolloids to overcome disadvantages posed by conventional antimicrobial agents. Hence, a novel, environmentally friendly, cost-effective, biocompatible, and long-term antibacterial represents a promising solution for medicine and farming. Hence, polyherbal nanocolloids (PHNc) was formulated by using the extracts of Syzygium aromaticum, Cinnamomum verum, Emblica officinalis, Terminalia belerica, Terminalia chebula, and Cymbopogon citratus and physicochemically characterized. From mastitis milk samples, microorganisms were isolated including Acinetobacter junii, Klebsiella pneumoniae, Pseudomonas stutzeri, and Acinetobacter baumannii and screened for antibiotic susceptibility. All the isolated strains were tested with PHNc and compared with antibiotics. Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and biofilm assays were performed at different concentrations, and antibacterial effects were quantified. In our results, PHNc showed potent bacteriostatic, bactericidal, and antibiofilm activity against all the strains. Our results indicated that PHNc can reduce the virulence factors responsible for infection by different bacterial strains. This study confirmed that PHNc had the potential to inhibit the growth of pathogenic Gram-negative and Gram-positive strains and could be utilized as an alternative to antibiotics to inhibit multidrug-resistant microbial pathogens in cattle.

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Funding

Authors are thankful to B.S. Abdur Rahman Institute of Science & Technology, Chennai for providing research facilities in school of life sciences. The authors also gratefully acknowledge the Ministry of Science and Technology, Department of Science and Technology (KIRAN Division) (GoI), New Delhi. (Ref No. DST/WOS-B/2018/1583-HFN (G)) and ASEAN University network (AUN)/Southeast Asia Engineering Education Development Network (SEED)/Japan International Cooperation Agency (JICA) SPRAC (SN042/MI.KU/2020).

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  1. School of Life Sciences, B.S. Abdur Rahman Crescent Institute of Science and Technology, Vandalur, Chennai, 600048, India

    S. Ranjani, P. Shruthy Priya & S. Hemalatha

  2. CisGEN Biotech Discoveries Pvt Limited, Chennai, Tamil Nadu, India

    Maroudam Veerasami

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  1. S. Ranjani
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SH conceived and designed the research. SR and SPP conducted experiments. SH and MVM analyzed the data. All authors wrote the manuscript. The authors read and approved the manuscript.

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Correspondence to S. Hemalatha.

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Ranjani, S., Priya, P.S., Veerasami, M. et al. Novel Polyherbal Nanocolloids to Control Bovine Mastitis. Appl Biochem Biotechnol 194, 246–265 (2022). https://doi.org/10.1007/s12010-021-03748-w

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