Abstract
This study investigated the in vitro potential of the Bidens pilosa as an antibiofilm-antimicrobial against Staphylococcus aureus. This pathogen is the most common causative agent of bovine mastitis, a disease responsible for economic losses in dairy production worldwide. Three extracts were obtained by macerating B. pilosa shoots in 70% ethanol for 4 h, 48 h, or 7 days (BPE4h, BPE48h, BPE7d). BPE4h and BPE48h had higher (p < 0.05) total phenolic and flavonoid contents than BPE7d. The antioxidant activities of BPE4h, BPE48h, and BPE7d were 80.7, 79.5, and 56.3%, respectively. Chromatographic analysis of extracts after solid-phase extraction suggested that flavonols were the major compounds in BPE4h and BPE48h and phenolic acids in BPE7d. Despite their chemical differences, all extracts showed antimicrobial activity against S. aureus (MIC = 0.3 mg/mL). BPE4h at 2 mg/mL completely inhibited and eradicated bacterial biofilms. The IC50 of BPE4h for MAC-T cells was 0.156 mg/mL. Our results show the great potential of B. pilosa as an ecofriendly and low-cost antimicrobial for sanitization of the milking environment for use in organic and agroecological dairy farms.
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This study was funded by Conselho Nacional de Desenvolvimento Científico e Tecnológico/CNPq (Edital 39/2013, 403415/2013–6).
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ILP, AAZ, and SK: conceptualization, data curation, methodology, validation, writing—original draft, review, and editing. ILP: laboratory work; SK: funding acquisition, project administration, writing—review and editing.
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Piccinin, I.N., Zielinski, A.A.F. & Kuhnen, S. Invasive plant Bidens pilosa as an ecofriendly antibiofilm-antimicrobial against Staphylococcus aureus for bovine mastitis control. Org. Agr. 13, 73–82 (2023). https://doi.org/10.1007/s13165-022-00415-0
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DOI: https://doi.org/10.1007/s13165-022-00415-0