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Elicitation Effects on Some Secondary Metabolites and Antioxidant Activity in Callus Cultures of Allium jesdianum Boiss. & Buhse.: Methyl Jasmonate and Putrescine

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Abstract

Allium jesdianum Boiss. & Buhse. is the most important species of the Amaryllidaceae family with various pharmacological properties. Three subsequent experiments (germination, callogenesis, and elicitation) were carried out as a completely randomized design with six replication. At the first study, the highest seed germination (78.33%) was achieved at chemical pre-treatment including the combination of α-naphthalene acetic acid (1 mg L−1) and benzylaminopurine (3 mg L−1) under in vitro condition. The highest callus induction (86.7%) was observed at MS/2 media, which was supplemented by NAA (1 mg L−1) and BAP (3 mg L−1) from hypocotyl explants. Then, two chemical elicitors including methyl jasmonate (MeJ) (0, 25, 50, and 100 µM) and putrescine (Pu) (0, 0.5, and 1 mM) were used to investigate their effects on different biochemical traits under callus culture. The results showed the superiority of MeJ over Pu for increasing the secondary metabolites and antioxidant activity in calluses of Allium jesdianum, compared to the control. The highest contents for total phenolics (6.02 mg GAE g−1 FW), total flavonoids (0.52 mg QE g−1 FW), and total flavonols (0.39 mg QE g−1 FW) were observed under 50 µM of MeJ. Meanwhile, the highest value for anthocyanin (8.99 µ mol g−1 FW) was achieved at 25 µM of MeJ. The highest 2,2-diphenyl-1-picrylhydrazyl activities were observed at 50 and 100 µM of MeJ. Putrescine (0.5 mM) elicitation showed only superiority for callus growth rate (0.53 mm day−1). Enhancement of desired secondary metabolites at 50 µM MeJ could be suitable for future studies in biotechnological aspects of this medicinal plant.

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Acknowledgements

The authors would like to thank Research Institute for Biotechnology and Bioengineering, Isfahan University of Technology, Isfahan, Iran, for supporting the practical part of this work. The authors would also thanks from Mostafa Abdollahi Bakhtiari for helping us in experimental sections of elicitation section.

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Authors and Affiliations

  1. Department of Natural Resources, Isfahan University of Technology, 8415683111, Isfahan, Iran

    Esmat Yazdanian, Pooran Golkar & Mohammad Reza Vahabi

  2. Research Institute for Biotechnology and Bioengineering, Isfahan University of Technology, 8415683111, Isfahan, Iran

    Pooran Golkar

  3. Department of Plant and Animal Biology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran

    Marzieh Taghizadeh

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  1. Esmat Yazdanian
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Esmat Yazdanian done the experimental sections of the study. Dr. Pooran Golkar done the conceptualization, data analysis, material preparation, writing original draft, reviewing, and final editing. Under the supervision of Dr. Mohammad Reza Vahabi, conceptualization and experimental sections were done. Dr. Marzieh Taghizadeh contributed on traits measurement and writing original draft of the manuscript.

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Yazdanian, E., Golkar, P., Vahabi, M.R. et al. Elicitation Effects on Some Secondary Metabolites and Antioxidant Activity in Callus Cultures of Allium jesdianum Boiss. & Buhse.: Methyl Jasmonate and Putrescine. Appl Biochem Biotechnol 194, 601–619 (2022). https://doi.org/10.1007/s12010-021-03643-4

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