Skip to main content

Advertisement

SpringerLink
Log in

Elicitor-mediated enhancement of biomass, polyphenols, mangiferin production and antioxidant activities in callus cultures of Salacia chinensis L.

  • Original Article
  • Published:
3 Biotech Aims and scope Submit manuscript

Abstract

The present investigation aimed to improve callus biomass, polyphenolic content, biosynthesis of mangiferin and biological potential following application of different elicitor treatments for medicinally important Salacia chinensis L. The leaf-derived callus cultures were established on Murashige and Skoog’s (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D: 2.0 mg/l) and 6-benzylaminopurine (BAP: 1.5 mg/l). These cultures were treated with different elicitors viz. jasmonic acid (JA), methyl jasmonate (MeJA) and yeast extracts (YE). The highest calli biomass (five-fold increase within 4 weeks) was achieved when callus was treated with JA (75 µM). The callus obtained on MS medium supplemented with 2,4-D (2.0 mg/l), BAP (1.5 mg/l) and treated with JA (75 µM) displayed augmented values for total phenolics, flavonoids and mangiferin contents. Besides, same treatment elicits the calli for antioxidant properties as evaluated by 2,2-diphenyl-2-picrylhydrazyl (DPPH), ferric-reducing antioxidant power (FRAP) and metal chelating assays. This is the first report on the elicitation study in genus Salacia and, therefore, the discoveries suggested that, S. chinensis calli might be a perfect source for large-scale production of industrially important secondary metabolites. Concurrently data provide accumulated information demonstrating its prominent antioxidant effect revealing its potential without disturbing natural resources.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

Abbreviations

2,4-D:

2,4-Dichlorophenoxyacetic acid

BAP:

6-Benzylaminopurine

CE:

Callus extract

CPF:

Callus proliferation frequency

DMF:

N,N-Dimethyl formamide

DPPH:

2,2-Diphenyl-2-picrylhydrazyl

DW:

Dry weight

FRAP:

Ferric-reducing antioxidant power

FW:

Fresh weight

GAE:

Gallic acid equivalent

JA:

Jasmonic acid

MeJA:

Methyl jasmonate

MeOH:

Methanol

MS:

Murashige and Skoog’s medium

QE:

Quercetin equivalent

RP-HPLC:

Reversed-phase high-performance liquid chromatography

SBAE:

Steam bath-assisted extraction

SE:

Standard error

TFC:

Total flavonoid content

TPC:

Total phenolic content

TPTZ:

2,4,6-Tripyridyl-s-triazine

YE:

Yeast extract

References

  • Ahmad Z, Shahzad A, Sharma S (2019) Chitosan versus yeast extract driven elicitation for enhanced production of fragrant compound 2-hydroxy-4-methoxybenzaldehyde (2H4MB) in root tuber derived callus of Decalepis salicifolia (Bedd. ex Hook.f.) Venter. Plant Cell Tiss Org Cult 136(1):29–40

    Article  CAS  Google Scholar 

  • Al-Khayri JM, Naik PM (2020) Elicitor-induced production of biomass and pharmaceutical phenolic compounds in cell suspension culture of date palm (Phoenix dactylifera L.). Molecules 25:4669

    Article  CAS  Google Scholar 

  • Astello-Garcia MG, Robles-Martinez M, Barba-de la Rosa AP, Santos-Diaz MS (2013) Establishment of callus from Opuntia robusta Wendl., a wild and medicinal cactus, for phenolic compounds production. Afr J Biotechnol 12:3204–3207

    CAS  Google Scholar 

  • Bagnazari M, Mahesh MG, Saidi M, Kini KR, Prakash HS, Geetha N (2018) Evaluation of genetic stability using FRAPD markers as novel method along with antioxidant and antidiabetic properties of micropropagated Salacia chinensis L. Acta Physiol Plantarum 40:128

    Article  Google Scholar 

  • Benzie IFF, Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: the FRAP assay. Anal Biochem 239:70–76

    Article  CAS  Google Scholar 

  • Brand-Williams W, Cuvelier ME, Berset C (1995) Use of free radical method to evaluate antioxidant activity. Lebensm Wiss Technol 28:25–30

    Article  CAS  Google Scholar 

  • Chang C, Yang M, Wen H, Chen J (2002) Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal 10:178–182

    CAS  Google Scholar 

  • Chavan JJ, Jagtap UB, Gaikwad NB, Dixit GB, Bapat VA (2013) Total phenolics, flavonoids and antioxidant activity of Saptarangi (Salacia chinensis L.) fruit pulp. J Plant Biochem Biotechnol 22(4):409–413

    Article  CAS  Google Scholar 

  • Chavan JJ, Ghadage DM, Bhoite AS, Umdale SD (2015a) Micropropagation, molecular profiling and RP-HPLC determination of mangiferin across various regeneration stages of Saptarangi (Salacia chinensis L.). Ind Crop Prod 76:1123–1132

    Article  CAS  Google Scholar 

  • Chavan JJ, Ghadage DM, Kshirsagar PR, Kudale SS (2015b) Optimization of extraction techniques and RP-HPLC analysis of antidiabetic and anticancer drug mangiferin from roots of ‘Saptarangi’ (Salacia chinensis L.). J Liq Chromato Reld Technol 38:963–969

    Article  CAS  Google Scholar 

  • Chavan JJ, Gaikwad NB, Dixit GB, Yadav SR, Bapat VA (2018) Biotechnological interventions for propagation, conservation and improvement of ‘Lantern Flowers’ (Ceropegia spp.). S Afr J Bot 114:192–216

    Article  CAS  Google Scholar 

  • Deepak KGK, Suneetha G, Surekha C (2015) In vitro clonal propagation of Salacia oblonga Wall. An endangered medicinal plant. Ann Phytomed 4(2):67–70

    CAS  Google Scholar 

  • Dhanasri G, Reddy M, Naresh B, Cherku D (2013) Micropropagation of Salacia reticulate—an endangered medicinal plant. Plant Tiss Cult Biotechnol 23(2):221–229

    Article  Google Scholar 

  • Dinis T, Madeira V, Almeida T (1994) Action of phenolic derivatives (acetaminophen, salicylate and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and peroxyl radical scavengers. Arch Biochem Biophys 315:161–165

    Article  CAS  Google Scholar 

  • Dubey GP, Agarwal A, Vyas N, Rajamanickam VG (2011) Herbal formulation for the prevention and management of diabetes mellitus and diabetic micro-vascular complications (US20090214678A1)

  • Espinosa-Leal CA, Puente-Garza CA, Garcia-Lara S (2018) In vitro plant tissue culture: means for production of biological active compounds. Planta 248:1–18

    Article  CAS  Google Scholar 

  • Feng ST, Wang ZZ, Yuan YH, Sun HM, Chen NH, Zhang Y (2019) Mangiferin: a multipotent natural product preventing neurodegeneration in Alzheimer’s and Parkinson’s disease models. Pharmacol Res 146:104336

    Article  CAS  Google Scholar 

  • Gao J, Xue J, Xue Y, LiuR RX, Wang S, Zhang X (2020) Transcriptome sequencing and identification of key callus browning-related genes from petiole callus of Tree Peony (Paeonia suffruticosa cv. Kao) cultured on media with three browning inhibitors. Plant Physiol Biochem 149:36–49

    Article  CAS  Google Scholar 

  • Ghadage DM, Kshirsagar PR, Pai SR, Chavan JJ (2017) Extraction efficiency, phytochemical profiles and antioxidative properties of different parts of Saptarangi (Salacia chinensis L.)—an important underutilized plant. Biochem Biophy Rep 12:79–90

    Google Scholar 

  • Jauhari N, Bharadwaj R, Sharma N, Bharadvaja N (2019) Assessment of bacoside production, total phenol content and antioxidant potential of elicited and non-elicited shoot cultures of Bacopa monnieri (L.). Environ Sustain 2:441–453

    Article  CAS  Google Scholar 

  • Khan T, Khan T, Hano C, Abbas BH (2019) Effects of chitosan and salicylic acid on the production of pharmacologically attractive secondary metabolites in callus cultures of Fagonia indica. Ind Crop Prod 129:525–535

    Article  CAS  Google Scholar 

  • Laxmi M, Raviraja Shetty G, Souravi K, Rajasekharan PE (2018) In vitro conservation studies in Salacia chinensis L. a threatened medicinal plant. J Pharmacog Phytochem SP3:78–81

    Google Scholar 

  • Mahendra C, Murali M, Manasa G, Sudarshana MS (2020) Biopotentiality of leaf and leaf derived callus extracts of Salacia macrosperma Wight.—an endangered medicinal plant of Western Ghats. Ind Crop Prod 143:111921

    Article  Google Scholar 

  • Majid BN, Kini KR, Prakash HS, Geetha N (2016a) Phytomorphology, phytochemistry and pharmacological activities of Salacia chinensis L., an endangered antidiabetic medicinal plant: a comprehensive review. Int J Agric Biosci 5(1):1–7

    Google Scholar 

  • Majid BN, Sampath KK, Prakash HS, Geetha N (2016b) Rapid mass propagation of Salacia chinensis L., an endangered valuable medicinal plant through direct organogenesis. Ind J Sci Technol 9(4):1–8

    CAS  Google Scholar 

  • Mekky H, Al-Sabahi J, Abdel-Kreem MFM (2018) Potentiating biosynthesis of the anticancer alkaloids vincristine and vinblastine in callus cultures of Catharanthus roseus. S Afr J Bot 114:29–31

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Rajan M, Feba KS, Chandran V, Shahena S, Mathew L (2020) Enhancement of rhamnetin production in Vernonia anthelmintica (L.) Willd. cell suspension cultures by eliciting with methyl jasmonate and salicylic acid. Physiol Mol Biol Plants 26:1531–1539

    Article  CAS  Google Scholar 

  • Ram M, Prasad KV, Singh SK, Hada BS, Kumar S (2013) Influence of salicylic acid and methyl jasmonate elicitation on anthocyanin production in callus cultures of Rosa hybrida L. Plant Cell Tiss Org Cult 113:459–467

    Article  CAS  Google Scholar 

  • Rao SR, Ravishankar GA (2002) Plant cell cultures: chemical factories of secondary metabolites. Biotechnol Adv 20(2):101–153

    Article  CAS  Google Scholar 

  • Sailo L, Upadhya V, Naik PM, Desai N, Pai SR, Al-Khayri JM (2018) Effect of chemical elicitors on pentacyclic triterpenoid production in in vitro cultures of Achyranthes aspera L. In: Kumar N (ed) Biotechnological approaches for medicinal and aromatic plants. Singapore, Springer Nature Singapore Pte Ltd

    Google Scholar 

  • Salma U, Kundu S, Ali MN, Mandal N (2018) Elicitor mediated enhancement of wedelolactone in cell suspension culture of Eclipta alba (L.) Hassk. Plant Cell Tiss Org Cult 134(3):409–421

    Article  CAS  Google Scholar 

  • Sarmadi M, Karimi N, Palazon J, Ghassempour A, Mirjalili MH (2018) The effects of salicylic acid and glucose on biochemical traits and taxane production in a Taxus baccata callus culture. Plant Physiol Biochem 132:271–280

    Article  CAS  Google Scholar 

  • Silpraist K, Seetaha S, Pongsanarakul P, Hannongbua S, Choowongkomon K (2011) Anti-HIV-1reverse transcriptase activities of hexane extracts from Asian medicinal plants. J Med Plants Res 5(19):4899–4906

    Google Scholar 

  • Singleton VL, Rossi JA (1965) Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents. Am J Enol Viticult 16:144–158

    CAS  Google Scholar 

  • Smetanska I (2008) Production of secondary metabolites using plant cell cultures. Adv Biochem Eng Biotechnol 111:187–228

    CAS  PubMed  Google Scholar 

  • Thuan LC (2005) Chemistry of Southeast Asian plants. Ph.D. thesis, National University of Singapore

  • Vijayalakshmi U, Shourie A (2019) Yeast extract-mediated elicitation of anti-cancerous compounds licoisoflavone B, licochalcone A, and liquirtigenin in callus cultures of Glycyrrhiza glabra. Biotechnologia 100(4):441–451

    Article  CAS  Google Scholar 

  • Yogananth N, Bhakyaraj R, Syed Ali M, Muthezhilan R (2019) Effect of yeast elicitor on the enhancement of kaempferol from in vivo and in vitro callus cultures of Dregea volubilis Benth. Asian Journal of Biological Sciences 12:278–283

    Article  CAS  Google Scholar 

  • Yoshikawa M, Nishida N, Shimoda H, Takada M, Kawahara Y, Matsuda H (2001) Polyphenol constituents from Salacia species: quantitative analysis of mangiferin with alpha-glucosidase and aldose reductase inhibitory activities. Yakugaku Zasshi 121:371–378

    Article  CAS  Google Scholar 

  • Zafar N, Mujib A, Ali M, Tonk D, Gulzar B (2017) Aluminum chloride elicitation (amendment) improves callus biomass growth and reserpine yield in Rauvolfia serpentine leaf callus. Plant Cell Tiss Org Cult 130(2):357–368

    Article  CAS  Google Scholar 

Download references

Acknowledgements

Authors are grateful to Rashtriya Uchchtar Shiksha Abhiyan (RUSA), MHRD, Govt. of India, New Delhi for providing funds for creation of high-end research facility under component 8. We extend our sincere gratitude towards the Head, Department of Botany and the Director, Yashavantrao Chavan Institute of Science, Satara (Autonomous) for providing necessary laboratory facilities.

Funding

The present work was financially supported by Science and Engineering Research Board (SERB) of Department of Science and Technology (DST), Govt. of India, New Delhi (No. SB/FT/LS-259/2012) through Fast-Track Scheme for Young Scientist.

Author information

Authors and Affiliations

  1. Department of Botany, Yashavantrao Chavan Institute of Science (Autonomous), Satara, 415 001, India

    Jaykumar J. Chavan

  2. Department of Biotechnology, Shivaji University, Kolhapur, 416004, India

    Parthraj R. Kshirsagar

  3. Department of Biotechnology, Yashavantrao Chavan Institute of Science (Autonomous), Satara, 415 001, India

    Jaykumar J. Chavan, Sharad G. Jadhav, Virdhaval M. Nalavade & Swati T. Gurme

  4. Department of Botany, Dada Patil Mahavidyalaya, Karjat, 414402, India

    Sandeep R. Pai

Authors
  1. Jaykumar J. Chavan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Parthraj R. Kshirsagar
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sharad G. Jadhav
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Virdhaval M. Nalavade
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Swati T. Gurme
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Sandeep R. Pai
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

JJC conceived, designed and performed the experiments, wrote the draft of manuscript. PRK, SGJ, VMN, STG performed the experiments, contributed to review and edit the manuscript. SRP contributed to resources and analyzed the data.

Corresponding author

Correspondence to Jaykumar J. Chavan.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Chavan, J.J., Kshirsagar, P.R., Jadhav, S.G. et al. Elicitor-mediated enhancement of biomass, polyphenols, mangiferin production and antioxidant activities in callus cultures of Salacia chinensis L.. 3 Biotech 11, 285 (2021). https://doi.org/10.1007/s13205-021-02836-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s13205-021-02836-2

Keywords

Search

Navigation