Abstract
One of the targets of the Sustainable Development Goals is clean and affordable energy. This is also the aim of the Biofuels Act of 2007 in the Philippines. However, this law is confronted with challenges such as the limitation of lignocellulosic feedstock, specifically available for bioethanol production. The present study sought to address the issue by exploring the potential of mango seed husk (MSH), a by-product of the mango industry, in bioethanol production. MSH is considered a waste material and its utilization also permit value-addition as this can serve as an alternative and affordable source of feedstock in energy production. Two pretreatment strategies are employed to exploit the cellulose and hemicellulose content of MSH, namely, dilute acid treatment and enzymatic hydrolysis. Results show that the %H2SO4 resulting in the highest glucose concentration and yield is 4% v/v at 95 °C hydrolysis temperature, 1:10 (w/v) solid-to-solvent ratio, and 60-min hydrolysis time. For enzymatic hydrolysis using a commercial enzyme preparation, the reaction time up to 72 h did not affect glucose concentration and yield at the following conditions: 50 °C hydrolysis temperature, 150 rpm, pH 5.0, 10% solids loading, and 4% enzyme loading. This could be attributed to the lignin and non-structural compounds present in MSHs. However, a combined process strategy of dilute acid pretreatment followed by enzymatic hydrolysis in the pretreatment of MSH contributes to an increased concentration and yield of sugars in the hydrolysates, which is advantageous for bioethanol production.
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All data generated or analyzed during this study are available from the corresponding author on reasonable request.
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Acknowledgments
The authors are genuinely indebted to the Department of Science and Technology—Engineering Research and Development for Technology (ERDT) for F.D.C.S.’s scholarship and research grant. Likewise, Novozyme A/S, Denmark is acknowledged for the enzyme samples. The authors would also like to recognize Ms. Pearly Jane Mendoza and Mr. Marvin Comendador for invaluable assistance during the conduct of this research.
Funding
This work was supported by the Department of Science and Technology—Engineering Research and Development for Technology (ERDT) program through the scholarship granted to F.D.C.S.
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Conceptualization: F.D.C.S., C.F.Y.L., and E.B.T. Methodology: F.D.C.S. Formal analysis and investigation: F.D.C.S. Writing—original draft preparation: F.D.C.S. Writing—review and editing: F.D.C.S., C.F.Y.L., and E.B.T. Funding acquisition: F.D.C.S., C.F.Y.L., and E.B.T. Resources: F.D.C.S., C.F.Y.L., and E.B.T. Supervision: F.D.C.S., C.F.Y.L., and E.B.T. All authors read and approved the final manuscript.
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Highlights
• Mango seed husk as a lignocellulosic material is a potential feedstock for bioethanol production.
• Chemical and enzyme-assisted pretreatment of mango seed husk generated sugar-rich hydrolysates.
• Dilute acid pretreatment using 4% H2SO4 at 95 °C for 1 h produces sugar-rich hydrolysates with minimum inhibitor content.
• Enzyme hydrolysis using a cellulase mixture at 50 °C for up to 72 h resulted in a hydrolysate having optimal sugar concentration.
• The combination of both dilute acid and enzymatic hydrolysis in the pretreatment process of mango seed husks significantly increased the sugar concentration of the resulting hydrolysates.
The presenting author in ACB2019 is F.D.C.S.
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Siacor, F.D.C., Lobarbio, C.F.Y. & Taboada, E.B. Pretreatment of Mango (Mangifera indica L. Anacardiaceae) Seed Husk for Bioethanol Production by Dilute Acid Treatment and Enzymatic Hydrolysis. Appl Biochem Biotechnol 193, 1338–1350 (2021). https://doi.org/10.1007/s12010-020-03387-7
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DOI: https://doi.org/10.1007/s12010-020-03387-7