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Comparative Transcriptome Analysis of Recombinant Industrial Saccharomyces cerevisiae Strains with Different Xylose Utilization Pathways

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Abstract

A heterologous xylose utilization pathway, either xylose reductase–xylitol dehydrogenase (XR–XDH) or xylose isomerase (XI), is usually introduced into Saccharomyces cerevisiae to construct a xylose-fermenting strain for lignocellulosic ethanol production. To investigate the molecular basis underlying the effect of different xylose utilization pathways on the xylose metabolism and ethanol fermentation, transcriptomes of flocculating industrial strains with the same genetic background harboring different xylose utilization pathways were studied. A different source of xylA did not obviously affect the change of the strains transcriptome, but compared with the XR–XDH strain, several key genes in the central carbon pathway were downregulated in the XI strains, suggesting a lower carbon flow to ethanol. The carbon starvation caused by lower xylose metabolism in XI strains further influenced the stress response and cell metabolism of amino acid, nucleobase, and vitamin. Besides, the downregulated genes mostly included those involved in mitotic cell cycle and the cell division–related process. Moreover, the transcriptomes analysis indicated that the after integrate xylA in the δ region, the DNA and chromosome stability and cell wall integrity of the strains were affected to some extent. The aim of this was to provide some reference for constructing efficient xylose-fermenting strains.

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Funding

This work was supported by the National Natural Science Foundation of China (31170093), the Talent Project for Science and Technology Innovation of Sichuan Province (2017RZ0021), and the Open Fund of Key Laboratory of Development and Application of Rural Renewable Energy, Ministry of Agriculture of China (2018-009).

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Authors and Affiliations

  1. College of Pharmacy and Biological Engineering, Chengdu University, No. 2025, Chengluo Road, Chengdu, 610065, China

    Yun-Cheng Li

  2. College of Architecture and Environment, Sichuan University, No. 24, South Section 1, First Ring Road, Chengdu, 610065, China

    Yun-Cheng Li, Cai-Yun Xie, Bai-Xue Yang, Yue-Qin Tang, Zhao-Yong Sun, Min Gou & Zi-Yuan Xia

  3. Key Laboratory of Development and Application of Rural Renewable Energy, Ministry of Agriculture, CAAS, Section 4-13, Renmin Nan Road, Chengdu, 610041, China

    Bo Wu

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Correspondence to Yue-Qin Tang.

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Li, YC., Xie, CY., Yang, BX. et al. Comparative Transcriptome Analysis of Recombinant Industrial Saccharomyces cerevisiae Strains with Different Xylose Utilization Pathways. Appl Biochem Biotechnol 189, 1007–1019 (2019). https://doi.org/10.1007/s12010-019-03060-8

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