Abstract
Novel synthetic isoprenoids have been synthesized in engineered microbial hosts by evolving terpene synthase or expressing heterologous terpene synthases. Recently, the native operon, crtNaNcM derived from Planococcus sp. PAMC 21323, has isolated for potential industrial applications of C35 carotenoids. For the first time, novel C35 carotenoids (sesquarterpene) were synthesized in Corynebacterium glutamicum expressing the crtNaNcM genes. The recombinant strains accumulate various sesquarterpene including 4-apolycopene (red color), 4-aponeurosporene (yellow color), and no pigmentation, depending on the expression of the genetic elements of the crtNaNcM genes. Subsequently, the carotenoid extract from the cells harboring pCES-H36-CrtNaNcM was analyzed, resulting in significantly higher antioxidant activity than those of other strains harboring pCES-H36-CrtNcM and pCES-H36-CrtNaNc, respectively. This study will promote further engineering of C. glutamicum to increase sesquarterpene productions.
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Acknowledgements
The authors would liketo thank Dr. Ki Jun Jeong at KAIST for providing a pCES-H36-GFP vector.
Funding
This study was financially supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (No.NRF-2015R1A2A2A01004733), by Golden Seed Project (213008-05-2-SB910), Ministry of Agriculture, Ministry of Oceans and Fisheries.
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Ravikumar, S., Woo, H.M. & Choi, Ji. Analysis of Novel Antioxidant Sesquarterpenes (C35 Terpenes) Produced in Recombinant Corynebacterium glutamicum. Appl Biochem Biotechnol 186, 525–534 (2018). https://doi.org/10.1007/s12010-018-2756-9
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DOI: https://doi.org/10.1007/s12010-018-2756-9