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A New Hydrogen-Producing Strain and Its Characterization of Hydrogen Production

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Abstract

A newly isolated photo non-sulfur (PNS) bacterium was identified as Rhodopseudomonas palustris PB-Z by sequencing of 16S ribosomal DNA (rDNA) genes and phylogenetic analysis. Under vigorous stirring (240 rpm), the hydrogen production performances were greatly improved: The maximum hydrogen production rate and cumulative hydrogen production increased by 188.9 ± 0.07 % and 83.0 ± 0.06 %, respectively, due to the hydrogen bubbles were immediately removed from the culture medium. The effects of different wavelength of light on hydrogen production with stirring were much different from that without stirring. The ranking on the photo-hydrogen production performance was white > yellow > green > blue > red without stirring and white > yellow > blue > red > green under stirring. The best light source for hydrogen production was tungsten filament lamp. The optimum temperature was 35 °C. The maximal hydrogen production rate and cumulative hydrogen production reached 78.7 ± 2.3 ml/l/h and 1728.1 ± 92.7 mol H2/l culture, respectively, under 35 °C, 240 rpm, and illumination of 4000 lux. Pyruvate was one of the main sources of CO2 and has a great impact on the gas composition.

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Acknowledgments

This work was supported by the International Cooperation Projects of Shanxi Province (No. 2013081020).

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Authors and Affiliations

  1. College of Chemistry and Chemical Engineering, Taiyuan University of Technology, Taiyuan, Shanxi, 030024, China

    Mingxing Sun

  2. Key Laboratory of Coal Science and Technology of Shanxi Province and Ministry of Education, Taiyuan University of Technology, Taiyuan, Shanxi, 030024, China

    Yongkang Lv

  3. College of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan, Shanxi, 030024, China

    Yuxiang Liu

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  1. Mingxing Sun
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Correspondence to Yongkang Lv.

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Sun, M., Lv, Y. & Liu, Y. A New Hydrogen-Producing Strain and Its Characterization of Hydrogen Production. Appl Biochem Biotechnol 177, 1676–1689 (2015). https://doi.org/10.1007/s12010-015-1845-2

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  • DOI: https://doi.org/10.1007/s12010-015-1845-2

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